Deng Xiangxiong, Zhang Su, Qing Quan, Wang Pengfei, Ma Haiyang, Ma Qinghua, Zhao Weixiang, Tang Hanjing, Lu Min
Zhejiang Gene Stem Cell Biotech Co. Ltd., Huzhou, Zhejiang, 313000, China.
Huzhou Maternity and Child Health Care Hospital, Huzhou, Zhejiang, 313000, China.
Biochem Biophys Rep. 2024 Jun 13;39:101739. doi: 10.1016/j.bbrep.2024.101739. eCollection 2024 Sep.
Mesenchymal stem cells (MSCs) have tremendous potential in cell therapy and regenerative medicine. The placenta-derived MSCs (PMSCs) are becoming favorable sources as they are ethically preferable and rich in MSCs. Although several subgroups of PMSCs have been identified from human term placenta, optimal sources for specific clinical applications remain to be elucidated. This study aimed to isolate MSCs from various components of the placenta, and compare their biological characteristics, including morphology, proliferation, immunophenotype, differentiation potential, growth factor and cytokine secretion, and immunomodulatory properties. Finally, four distinct groups of PMSCs were isolated from the placenta: amniotic membrane-derived MSCs (AM-MSCs), chorionic membrane-derived MSCs (CM-MSCs), chorionic plate-derived MSCs (CP-MSCs), and chorionic villi-derived MSCs (CV-MSCs). The results showed that CV-MSCs had good proliferation ability, and were easier to induce osteogenic and chondrogenic differentiation; CP-MSCs exhibited the strongest inhibitory effect on the proliferation of activated T cells, secreted high levels of EGF and IL-6, and could well differentiate into osteoblasts, adipocytes, and chondroblasts; AM-MSCs showed good growth dynamics in the early generations, were able to grow at high density, and tended to induce differentiation into osteogenic and neural lineages. These findings may provide novel evidence for the selection of seed cells in clinical application.
间充质干细胞（MSCs）在细胞治疗和再生医学中具有巨大潜力。胎盘来源的间充质干细胞（PMSCs）因其伦理上更可取且富含间充质干细胞而成为有利的来源。尽管已从人足月胎盘中鉴定出几个PMSCs亚组，但特定临床应用的最佳来源仍有待阐明。本研究旨在从胎盘的各个成分中分离间充质干细胞，并比较它们的生物学特性，包括形态、增殖、免疫表型、分化潜能、生长因子和细胞因子分泌以及免疫调节特性。最后，从胎盘中分离出四组不同的PMSCs：羊膜来源的间充质干细胞（AM-MSCs）、绒毛膜来源的间充质干细胞（CM-MSCs）、绒毛板来源的间充质干细胞（CP-MSCs）和绒毛膜绒毛来源的间充质干细胞（CV-MSCs）。结果表明，CV-MSCs具有良好的增殖能力，且更容易诱导成骨和软骨分化；CP-MSCs对活化T细胞的增殖表现出最强的抑制作用，分泌高水平的表皮生长因子（EGF）和白细胞介素-6（IL-6），并能很好地分化为成骨细胞、脂肪细胞和成软骨细胞；AM-MSCs在早期传代中表现出良好的生长动态，能够高密度生长，并倾向于诱导分化为成骨和神经谱系。这些发现可能为临床应用中种子细胞的选择提供新的证据。
