Department of Chemistry and Biotechnology, School of Science, Computing & Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
Department of Pharmacology and Toxicology, College of Pharmacy, University of Ha'il, Ha'il 55211, Saudi Arabia.
Molecules. 2023 May 23;28(11):4258. doi: 10.3390/molecules28114258.
Herein, polydopamine (PDA)-based antimicrobial coatings loaded with silver nanoparticles (Ag NPs) and gentamicin were designed and prepared on glass slides using two different approaches. To our knowledge, this study was performed for the first time with the aim to compare these methods (viz., in situ loading and physical adsorption method) regarding the loading and release behavior of payloads. In one method, gentamicin was in situ loaded on PDA-coated substrates during PDA polymerization followed by Ag NPs immobilization (named as Ag@Gen/PDA); for the second method, Ag NPs and gentamicin were simultaneously loaded onto PDA via physical adsorption by immersing pre-formed PDA coatings into a mixed solution of Ag NPs and gentamicin (named as Ag/Gen@PDA). The loading and release characteristics of these antimicrobial coatings were compared, and both gave variable outcomes. The in situ loading method consequently provided a relatively slow release of loaded antimicrobials, i.e., approx. 46% for Ag@Gen/PDA as compared to 92% from physically adsorbed Ag/GenPDA in an immersion period of 30 days. A similar trend was observed for gentamicin release, i.e., ~0.006 µg/mL from Ag@Gen/PDA and 0.02 µg/mL from Ag/Gen@PDA each day. The slower antimicrobial release from Ag@Gen/PDA coatings would ultimately provide an effective long-term antimicrobial property as compared to Ag/Gen@PDA. Finally, the synergistic antimicrobial activities of these composite coatings were assessed against two microbial species, namely, and , hence providing evidence in the prevention of bacterial colonization.
在此，通过两种不同的方法，在载玻片上设计并制备了负载载银纳米粒子（Ag NPs）和庆大霉素的基于聚多巴胺（PDA）的抗菌涂层。据我们所知，这项研究首次旨在比较这些方法（即，原位负载和物理吸附方法）在负载和释放载药方面的性能。在一种方法中，在 PDA 聚合过程中，庆大霉素原位负载在 PDA 涂层上，随后固定 Ag NPs（命名为 Ag@Gen/PDA）；对于第二种方法，通过将预形成的 PDA 涂层浸入 Ag NPs 和庆大霉素的混合溶液中，同时将 Ag NPs 和庆大霉素物理吸附到 PDA 上（命名为 Ag/Gen@PDA）。比较了这些抗菌涂层的负载和释放特性，两种方法都给出了不同的结果。原位负载方法随后提供了负载抗菌剂的相对缓慢释放，即在 30 天的浸泡期内，Ag@Gen/PDA 约为 46%，而物理吸附的 Ag/Gen@PDA 为 92%。庆大霉素释放也观察到类似的趋势，即 Ag@Gen/PDA 每天约为 0.006 µg/mL，Ag/Gen@PDA 每天为 0.02 µg/mL。与 Ag/Gen@PDA 相比，Ag@Gen/PDA 涂层中较慢的抗菌剂释放最终将提供有效的长期抗菌性能。最后，评估了这些复合涂层对两种微生物物种，即 和 的协同抗菌活性，从而为预防细菌定植提供了证据。
