Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
Molecules. 2020 Apr 30;25(9):2090. doi: 10.3390/molecules25092090.
The mussel inspired polydopamine has acquired great relevance in the field of nanomedicines, owing to its incredible physicochemical properties. Polydopamine nanoparticles (PDA NPs) due to their low cytotoxicity, high biocompatibility and ready biodegradation have already been widely investigated in various drug delivery, chemotherapeutic, and diagnostic applications. In addition, owing to its highly reactive nature, it possesses a very high capability for loading drugs and chemotherapeutics. Therefore, the loading efficiency of PDA NPs for an antibiotic i.e., gentamicin (G) has been investigated in this work. For this purpose, an in-situ polymerization method was studied to load the drug into PDA NPs using variable drug: monomer ratios. Scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) confirmed the successful loading of drug within PDA NPs, mainly via hydrogen bonding between the amine groups of gentamicin and the hydroxyl groups of PDA. The loading amount was quantified by liquid chromatography-mass spectrometry (LC-MS) and the highest percentage loading capacity was achieved for G-PDA prepared with drug to monomer ratio of 1:1. Moreover, the gentamicin loaded PDA NPs were tested in a preliminary antibacterial evaluation using the broth microdilution method against both Gram-(+) and Gram-(-) microorganisms. The highest loaded G-PDA sample exhibited the lowest minimum inhibitory concentration and minimum bactericidal concentration values. The developed gentamicin loaded PDA is very promising for long term drug release and treating various microbial infections.
贻贝启发的聚多巴胺由于其令人难以置信的物理化学性质，在纳米医学领域具有重要意义。聚多巴胺纳米粒子（PDA NPs）由于其低细胞毒性、高生物相容性和可生物降解性，已广泛应用于各种药物输送、化学治疗和诊断应用中。此外，由于其高反应性，它具有非常高的载药能力。因此，本工作研究了 PDA NPs 对一种抗生素即庆大霉素（G）的载药效率。为此，研究了一种原位聚合方法，通过改变药物：单体比例将药物载入 PDA NPs 中。扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和 X 射线光电子能谱（XPS）证实了药物成功载入 PDA NPs 中，主要是通过庆大霉素的胺基与 PDA 的羟基之间的氢键。通过液相色谱-质谱（LC-MS）定量载药量，药物与单体比例为 1:1 时制备的 G-PDA 的载药量最高。此外，使用肉汤微量稀释法对负载庆大霉素的 PDA NPs 进行了初步的抗菌评估，以革兰氏阳性菌和革兰氏阴性菌为研究对象。负载量最高的 G-PDA 样品表现出最低的最小抑菌浓度和最小杀菌浓度值。开发的负载庆大霉素的 PDA 非常有前途，可用于长期药物释放和治疗各种微生物感染。
