Kawaguchi Akira, Kitabayashi Shoya, Inoue Koji, Tanina Koji
Western Region Agricultural Research Center (WARC) (Kinki, Chugoku, and Shikoku Regions), National Agriculture and Food Research Organization (NARO), Fukuyama 721-8514, Hiroshima, Japan.
Research Institute for Agriculture, Okayama Prefectural Technology Center for Agriculture, Forestry and Fisheries, Akaiwa 709-0801, Okayama, Japan.
Plants (Basel). 2023 May 25;12(11):2099. doi: 10.3390/plants12112099.
A pathogen, healthy, latently infected, infectious, and diseased plant (PHLID) model for botanical epidemics was defined for tomato bacterial canker (TBC) caused by the pathogenic plant bacteria, subsp. (). First, the incubation period had to be defined to develop this type of model. To estimate the parameter of incubation period, inoculation experiments were conducted in which it was assumed that infection is transferred to healthy plants by cutting with contaminated scissors after cutting infected plants with early symptoms or symptomless. The concentration of was increased over 1 × 10 cells/g plant tissue at 20 cm away from the inoculated point on the stem 10 days after inoculation, and then the approximate incubation period of TBC in symptomless infected plants was defined as 10 days. The developed PHLID model showed the dynamics of diseased plants incidence and fitted the curve of the proportion of diseased plants observed in fields well. This model also contains the factors of pathogen and disease control, and it was able to simulate the control effects and combined two different control methods, which were the soil and scissors disinfections to prevent primary and secondary transmissions, respectively. Thus, this PHLID model for TBC can be used to simulate not only the increasing number of diseased plants but also suppressing disease increase.
针对由致病植物细菌亚种（）引起的番茄细菌性溃疡病（TBC），定义了一种用于植物病害流行的病原体、健康、潜伏感染、感染性和患病植物（PHLID）模型。首先，必须定义潜伏期来建立这种类型的模型。为了估计潜伏期参数，进行了接种实验，假设在用早期有症状或无症状的感染植物切割后，用受污染的剪刀切割将感染传播给健康植物。接种后10天，在茎上距接种点20厘米处，植物组织中 的浓度增加到超过1×10个细胞/克，然后将无症状感染植物中TBC的近似潜伏期定义为10天。所建立的PHLID模型显示了患病植物发病率的动态变化，并很好地拟合了田间观察到的患病植物比例曲线。该模型还包含病原体和病害控制因素，并且能够模拟控制效果并结合两种不同的控制方法，即分别进行土壤和剪刀消毒以防止初次和二次传播。因此，这种TBC的PHLID模型不仅可用于模拟患病植物数量的增加，还可用于模拟抑制病害增加的情况。
