Lee Dong-Hwan, Son Yong-Hwan, Jang Jun-Hyuk, Lee Sun-Young, Kim Hyun-Jun
Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju-si 36040, Republic of Korea.
Plants (Basel). 2023 May 25;12(11):2107. doi: 10.3390/plants12112107.
is a traditional medicinal herb in East Asia. The compounds of plants vary depending on environmental factors, such as soil, temperature, drainage, and so on. However, few to no studies have been done on the correlation among environment, growth, and compounds in . Thus, we aimed to investigate their relationship. Samples of fruit and cultivation soil were collected from 28 cultivation sites in October 2021. Six growth characteristics, eleven soil physicochemical properties, seven meteorological data points, and three active compounds were investigated in this study. We developed and validated an optimized method for quantifying active compounds using UPLC and performed correlation analysis of the environment, growth characteristics, and active compounds. The UPLC-UV method for determining active compounds was validated by measuring the linearity, LOD, LOQ, precision, and accuracy using UPLC. The LOD and LOQ were 0.01-0.03 µg/mL and 0.04-0.09 µg/mL, respectively. The precision was acceptable with RSD% values less than 2%. The recoveries ranged from 97.25 to 104.98% with RSD values <2%, within the acceptable limits. The active compounds were negatively correlated with the size of the fruit, and the growth characteristics were negatively correlated with some environmental factors. The results of this study can be used as basic data for the standard cultural practices and quality control of fruits.
是东亚的一种传统草药。植物的化合物因环境因素而异,如土壤、温度、排水等。然而,关于[草药名称]环境、生长和化合物之间的相关性研究很少或几乎没有。因此,我们旨在研究它们之间的关系。2021年10月从28个种植地点采集了[草药名称]果实和种植土壤样本。本研究调查了六个生长特征、十一个土壤理化性质、七个气象数据点和三种活性化合物。我们开发并验证了一种使用超高效液相色谱法(UPLC)定量活性化合物的优化方法,并对环境、生长特征和活性化合物进行了相关性分析。通过使用UPLC测量线性、检测限(LOD)、定量限(LOQ)、精密度和准确度,验证了用于测定活性化合物的UPLC-UV方法。LOD和LOQ分别为0.01 - 0.03 µg/mL和0.04 - 0.09 µg/mL。精密度可接受,相对标准偏差(RSD%)值小于2%。回收率在97.25%至104.98%之间,RSD值<2%,在可接受范围内。活性化合物与果实大小呈负相关,生长特征与一些环境因素呈负相关。本研究结果可作为[草药名称]果实标准栽培实践和质量控制的基础数据。