Maslennikova Dilara, Ivanov Sergey, Petrova Svetlana, Burkhanova Guzel, Maksimov Igor, Lastochkina Oksana
Institute of Biochemistry and Genetics UFRC RAS, 71 Pr. Oktyabrya, 450054 Ufa, Russia.
Ufa Institute of Chemistry UFRC RAS, 69 Pr. Oktyabrya, 450054 Ufa, Russia.
Plants (Basel). 2023 May 26;12(11):2123. doi: 10.3390/plants12112123.
Nitric oxide (NO) is a multifunctional, gaseous signaling molecule implicated in both physiological and protective responses to biotic and abiotic stresses, including salinity. In this work, we studied the effects of 200 µM exogenous sodium nitroprusside (SNP, a donor of NO) on the components of the phenylpropanoid pathway, such as lignin and salicylic acid (SA), and its relationship with wheat seedling growth under normal and salinity (2% NaCl) conditions. It was established that exogenous SNP contributed to the accumulation of endogenous SA and increased the level of transcription of the pathogenesis-related protein 1 () gene. It was found that endogenous SA played an important role in the growth-stimulating effect of SNP, as evidenced by the growth parameters. In addition, under the influence of SNP, the activation of phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL), and peroxidase (POD), an increase in the level of transcription of the and genes, and the acceleration of lignin accumulation in the cell walls of roots were revealed. Such an increase in the barrier properties of the cell walls during the period of preadaptation played an important role in protection against salinity stress. Salinity led to significant SA accumulation and lignin deposition in the roots, strong activation of TAL, PAL, and POD, and suppression of seedling growth. Pretreatment with SNP under salinity conditions resulted in additional lignification of the root cell walls, decreased stress-induced endogenous SA generation, and lower PAL, TAL, and POD activities in comparison to untreated stressed plants. Thus, the obtained data suggested that during pretreatment with SNP, phenylpropanoid metabolism was activated (i.e., lignin and SA), which contributed to reducing the negative effects of salinity stress, as evidenced by the improved plant growth parameters.
一氧化氮（NO）是一种多功能气态信号分子，参与对生物和非生物胁迫（包括盐度）的生理和保护反应。在本研究中，我们研究了200 μM外源硝普钠（SNP，一种NO供体）对苯丙烷类途径成分（如木质素和水杨酸（SA））的影响，以及在正常和盐度（2% NaCl）条件下其与小麦幼苗生长的关系。结果表明，外源SNP促进了内源SA的积累，并提高了病程相关蛋白1（）基因的转录水平。生长参数表明，内源SA在SNP的生长促进作用中发挥了重要作用。此外，在SNP的影响下，苯丙氨酸解氨酶（PAL）、酪氨酸解氨酶（TAL）和过氧化物酶（POD）被激活，和基因的转录水平增加，并且根细胞壁中木质素积累加速。在预适应期，细胞壁屏障特性的这种增加在抵御盐胁迫中起重要作用。盐度导致根中SA大量积累和木质素沉积，TAL、PAL和POD强烈激活，以及幼苗生长受抑制。与未处理的胁迫植株相比，在盐度条件下用SNP预处理导致根细胞壁额外木质化，胁迫诱导的内源SA生成减少，以及PAL、TAL和POD活性降低。因此，获得的数据表明，在SNP预处理期间，苯丙烷类代谢被激活（即木质素和SA），这有助于减轻盐胁迫的负面影响，植株生长参数的改善证明了这一点。
