Mahlare Mary-Jane S, Husselmann Lizex, Lewu Muinat N, Bester Cecilia, Lewu Francis B, Caleb Oluwafemi James
Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Stellenbosch 7599, South Africa.
Department of Agriculture, Faculty of Applied Sciences, Cape Peninsula University of Technology, Wellington Campus, Private Bag X8, Wellington 7654, South Africa.
Plants (Basel). 2023 May 31;12(11):2181. doi: 10.3390/plants12112181.
Honeybush ( spp.) is a rich source of antioxidant properties and phenolic compounds. Water availability plays a crucial role in plant metabolic processes, and it contributes to overall quality. Thus, this study aimed to investigate changes in molecular functions, cellular components, and biological processes of exposed to different water stress conditions, which include well-watered (as Control, T1), semi-water stressed (T2), and water-deprived (T3) potted plants. Samples were also collected from a well-watered commercial farm first cultivated in 2013 (T13) and then cultivated in 2017 (T17) and 2019 (T19). Differentially expressed proteins extracted from leaves were identified using LC-MS/MS spectrometry. A total of 11 differentially expressed proteins (DEPs) were identified using Fisher's exact test ( < 0.00100). Only α-glucan phosphorylase was found to be statistically common between T17 and T19 ( < 0.00100). Notably, α-glucan phosphorylase was upregulated in the older vegetation (T17) and downregulated in T19 by 1.41-fold. This result suggests that α-glucan phosphorylase was needed in T17 to support the metabolic pathway. In T19, five DEPs were upregulated, while the other six were downregulated. Based on gene ontology, the DEPs in the stressed plant were associated with cellular and metabolic processes, response to stimulus, binding, catalytic activity, and cellular anatomical entity. Differentially expressed proteins were clustered based on the Kyoto Encyclopedia of Genes and Genomes (KEGG), and sequences were linked to metabolic pathways via enzyme code and KEGG ortholog. Most proteins were involved in photosynthesis, phenylpropanoid biosynthesis, thiamine, and purine metabolism. This study revealed the presence of trans-cinnamate 4-monooxygenase, an intermediate for the biosynthesis of a large number of substances, such as phenylpropanoids and flavonoids.
蜜 bush（多种）富含抗氧化特性和酚类化合物。水分供应在植物代谢过程中起着关键作用，并影响整体品质。因此，本研究旨在调查暴露于不同水分胁迫条件下的蜜 bush 的分子功能、细胞成分和生物学过程的变化，这些条件包括水分充足（作为对照，T1）、半水分胁迫（T2）和缺水（T3）的盆栽植物。样本还取自一个 2013 年首次种植（T13）、然后在 2017 年（T17）和 2019 年（T19）种植的水分充足的商业农场。使用液相色谱 - 串联质谱法（LC - MS/MS）鉴定从叶片中提取的差异表达蛋白质。使用 Fisher 精确检验（<0.00100）共鉴定出 11 种差异表达蛋白质（DEP）。仅发现 α - 葡聚糖磷酸化酶在 T17 和 T19 之间具有统计学上的共性（<0.00100）。值得注意的是，α - 葡聚糖磷酸化酶在较老的植被（T17）中上调，而在 T19 中下调了 1.41 倍。这一结果表明 T17 需要 α - 葡聚糖磷酸化酶来支持代谢途径。在 T19 中，5 种 DEP 上调，而其他 6 种下调。基于基因本体论，受胁迫植物中的 DEP 与细胞和代谢过程、对刺激的反应、结合、催化活性以及细胞解剖实体相关。差异表达蛋白质根据京都基因与基因组百科全书（KEGG）进行聚类，序列通过酶代码和 KEGG 直系同源物与代谢途径相连。大多数蛋白质参与光合作用、苯丙烷生物合成、硫胺素和嘌呤代谢。本研究揭示了反式肉桂酸 4 - 单加氧酶的存在，它是大量物质如苯丙烷类和黄酮类生物合成的中间体。
