Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
University of Chinese Academy of Sciences, Beijing 100008, China.
Nutrients. 2023 May 26;15(11):2476. doi: 10.3390/nu15112476.
Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals. Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction. This study evaluated the optimal dosage of OKG in healthy mice. Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota. Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period. Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NHL, and Ile. Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors. During days 14 to 21, the DSS mice were administered 4% DSS to induce colitis. The results revealed that OKG alleviated weight loss and reversed the increases in colonic histological damage induced by DSS. OKG also increased serum IL-10 secretion. Moreover, OKG enhanced the abundance of Firmicutes and decreased that of Bacteriodetes at the phylum level and particularly enhanced the abundance of Alistipes and reduced that of Parabacterioides at the genus level. Our results indicated that OKG promotes growth performance and hormone secretion and regulates serum biochemical indicators and amino acid concentrations. Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
鸟氨酸 α-酮戊二酸（OKG）是一种营养化合物，是一种具有抗氧化和抗炎作用的氨基酸盐，对人类和动物都有作用。溃疡性结肠炎（UC）作为一种炎症性肠病（IBD），导致慢性肠道炎症功能障碍。本研究评估了 OKG 在健康小鼠中的最佳剂量。然后，使用葡聚糖硫酸钠（DSS）建立了小鼠急性结肠炎模型，通过分析血清炎症细胞因子和粪便微生物群，探讨了 OKG 对 DSS 诱导的小鼠结肠炎的预防作用。首先，将小鼠随机分为对照组、低剂量 OKG 组（LOKG：0.5%）、中剂量 OKG 组（MOKG：1%）和高剂量 OKG 组（HOKG：1.5%）；在整个 14 天的实验期间，它们一直保持在这些组中。我们的结果表明，1% OKG 补充剂增加了体重、血清生长激素（GH）、胰岛素（INS）、碱性磷酸酶（ALP）、酪氨酸（Tyr）和组氨酸（His），降低了尿素氮（BUN）、NHL 和异亮氨酸（Ile）。然后，采用 2×2 因子设计，共 40 只小鼠，饮食（标准饮食或 1% OKG 饮食）和挑战（4% DSS 或不）为主要因素。在第 14 天至第 21 天，给予 DSS 小鼠 4% DSS 诱导结肠炎。结果表明，OKG 减轻了体重减轻，并逆转了 DSS 诱导的结肠组织学损伤的增加。OKG 还增加了血清 IL-10 的分泌。此外，OKG 增加了厚壁菌门的丰度，降低了拟杆菌门的丰度，特别是增加了 Alistipes 的丰度，降低了 Parabacterioides 的丰度。我们的结果表明，OKG 促进生长性能和激素分泌，调节血清生化指标和氨基酸浓度。此外，1% OKG 补充剂通过改变微生物组成和减少血清中炎症细胞因子的分泌来预防 DSS 诱导的小鼠结肠炎。
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