一种从外周血中富集和单细胞分析循环肿瘤细胞的微流控方法。
A Microfluidic Approach for Enrichment and Single-Cell Characterization of Circulating Tumor Cells from Peripheral Blood.
机构信息
School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, Australia.
Institute for Biomedical Materials and Devices (IBMD), University of Technology Sydney, Sydney, NSW, Australia.
出版信息
Methods Mol Biol. 2023;2679:141-150. doi: 10.1007/978-1-0716-3271-0_9.
The emergence of enabling technologies for the analysis of circulating tumor cells has been shedding new lights into cancer management in the recent years. However, majority of the technologies developed suffer from excessive cost, time-consuming workflows, and reliance on specialized equipment and operators. Herein, we propose a simple workflow for the isolation and characterization of single circulating tumor cells using microfluidic devices. The entire process can be operated by a laboratory technician without relying on any microfluidic expertise and can be completed within few hours of sample collection.
近年来，用于分析循环肿瘤细胞的使能技术的出现为癌症管理带来了新的曙光。然而，大多数开发的技术都存在成本过高、工作流程耗时且依赖于专用设备和操作人员等问题。在此，我们提出了一种使用微流控设备分离和表征单个循环肿瘤细胞的简单工作流程。整个过程可以由实验室技术人员操作，无需依赖任何微流控专业知识，并且可以在样本采集后的几个小时内完成。
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