Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Methods Mol Biol. 2023;2679:233-240. doi: 10.1007/978-1-0716-3271-0_16.
Liquid biopsies are examination procedures for deciding the grouping of malignant growth cells tracked down in samples of blood and other body fluids. Liquid biopsies are likewise significantly less intrusive than tissue biopsies as they just require small amount of blood or body fluids from the patient. With the utilization of microfluidics, cancer cells can be isolated from the fluid biopsy and achieve early diagnosis. 3D printing is turning out to be progressively well known for microfluidic devices creation. 3D printing has shown multiple advantages compared to traditional microfluidic devices production, including effortless large-scale manufacturing of precise copies, the fuse of new materials, and execution of additional complicated or drawn-out plans that are hard to execute in conventional microfluidic devices. Combining 3D printing with microfluidics makes for a relatively inexpensive analysis of liquid biopsies with a chip that can be more advantageous to use over traditional microfluidic chips. In this chapter, a method for affinity-based separation of cancer cells in a liquid biopsy using a 3D microfluidic chip will be discussed, along with the rationale behind the method.
液体活检是一种检查程序，用于确定在血液和其他体液样本中发现的恶性肿瘤细胞的分组。液体活检也比组织活检侵入性更小，因为它们只需要从患者身上抽取少量的血液或体液。通过使用微流控技术，可以从液体活检中分离出癌细胞，实现早期诊断。3D 打印在微流控器件的制造中变得越来越流行。3D 打印与传统的微流控器件制造相比具有多种优势，包括可以轻松地大规模制造精确的复制品、融合新材料以及执行传统微流控器件难以执行的更复杂或耗时的计划。将 3D 打印与微流控技术相结合，使得使用芯片对液体活检进行相对廉价的分析成为可能，这种芯片比传统的微流控芯片更便于使用。在本章中，将讨论使用 3D 微流控芯片基于亲和力的液体活检中癌细胞的分离方法，以及该方法的基本原理。
