Environmental Engineering Department, College of Engineering, University of Baghdad, Baghdad, Iraq.
Iraqi Center for Cancer and Medical Genetics Research, Al Mustansiriyah University, Baghdad, Iraq.
Lasers Med Sci. 2023 Jun 10;38(1):135. doi: 10.1007/s10103-023-03796-0.
Pulsed laser ablation in liquids (PLAL) is considered as green, cost effective, and facile method to produce nanocolloids which exhibit anticancer effect. When comparing breast cancer with other types of cancers, breast cancer is considered as the second cause of death in women. The objective of this article is to test the cytotoxicity of carbon-based materials prepared by PLAL on both the normal (REF) cell line and the human breast cancer (MCF7) cell line. In this study, PLAL is used to prepare nanocolloids of asphalt and coal in different solvents (ethanol, dimethyl sulfoxide (DMSO), phosphate buffer saline (PBS), and distilled water (DW)). A fiber laser of wavelength of 1.06 μm and an average power of 10 watts was used to prepare different nanocolloids in different solvents from asphalt and coal. The cytotoxic effect of the prepared materials was tested against breast cancer MCF7 cell line in vitro. The asphalt in both ethanol and DMSO was found to have a significant cytotoxic effect and the growth inhibition (GI) was found to be 62.1% and 50.5% at concentrations of 620 and 80 ppm respectively, unlike the coal in DMSO which showed G.I. of 59.5%. Both the prepared materials in the mentioned solvents showed low cytotoxicity against the normal cell line (REF). We can conclude that the organic materials prepared in organic solvents using the PLAL had shown a low cytotoxicity against the (REF) cell line while they exhibited a significant cytotoxic effect against the MCF7 cell line. Further studies are recommended to test these prepared materials in vivo.
脉冲激光烧蚀在液体中（PLAL）被认为是一种绿色、经济高效且简便的方法，可以制备出具有抗癌作用的纳米胶体。在将乳腺癌与其他类型的癌症进行比较时，乳腺癌被认为是女性死亡的第二大原因。本文的目的是测试通过 PLAL 制备的碳基材料对正常（REF）细胞系和人乳腺癌（MCF7）细胞系的细胞毒性。在这项研究中，PLAL 用于在不同溶剂（乙醇、二甲基亚砜（DMSO）、磷酸盐缓冲盐水（PBS）和去离子水（DW））中制备沥青和煤的纳米胶体。使用波长为 1.06 μm 和平均功率为 10 瓦的光纤激光器，从沥青和煤中在不同溶剂中制备不同的纳米胶体。测试了制备材料对体外乳腺癌 MCF7 细胞系的细胞毒性作用。发现在乙醇和 DMSO 中的沥青具有显著的细胞毒性作用，在浓度为 620 和 80 ppm 时，生长抑制（GI）分别为 62.1%和 50.5%，而在 DMSO 中的煤则显示 GI 为 59.5%。在所述溶剂中制备的两种材料对正常细胞系（REF）的细胞毒性均较低。我们可以得出结论，使用 PLAL 在有机溶剂中制备的有机材料对（REF）细胞系表现出低细胞毒性，而对 MCF7 细胞系表现出显著的细胞毒性作用。建议进行进一步的研究，以测试这些在体内制备的材料。
