Chen Junqing, Peng Zihao, Ji Min, Wang Peng
School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu 211189, PR China.
School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu 211189, PR China.
Bioorg Chem. 2023 Sep;138:106655. doi: 10.1016/j.bioorg.2023.106655. Epub 2023 Jun 7.
Sulfatase participates in a variety of physiological processes in organisms including hormone regulation, cell signaling, and bacterial pathogenesis. Current sulfatase fluorescent probes can be used to track sulfate esterase overexpression in cancer cells for diagnostic purposes and to understand the pathological activity of sulfate esterase. However, some sulfatase fluorescent probes based on the hydrolysis of the sulfate bond were easily disturbed by the catalytic activity of sulfatase. Herein, we developed the fluorescent probe BQM-NH for sulfatase detection, which was based on the quinoline-malononitrile. The probe BQM-NH showed a fast response to sulfatase within 1 min and satisfactory sensitivity with a calculated LOD of 1.73 U/L. Importantly, it was successfully used to monitor endogenous sulfate in tumor cells, indicating BQM-NH has the potential to monitor sulfatase under physiological and pathological conditions.
硫酸酯酶参与生物体中的多种生理过程，包括激素调节、细胞信号传导和细菌发病机制。目前的硫酸酯酶荧光探针可用于追踪癌细胞中硫酸酯酶的过表达以进行诊断，并了解硫酸酯酶的病理活性。然而，一些基于硫酸酯键水解的硫酸酯酶荧光探针容易受到硫酸酯酶催化活性的干扰。在此，我们开发了基于喹啉-丙二腈的用于检测硫酸酯酶的荧光探针BQM-NH。探针BQM-NH在1分钟内对硫酸酯酶表现出快速响应，灵敏度令人满意，计算得到的检测限为1.73 U/L。重要的是，它成功用于监测肿瘤细胞中的内源性硫酸盐，表明BQM-NH有潜力在生理和病理条件下监测硫酸酯酶。
