基于硫酸酯酶激活荧光团的分枝杆菌种属和菌株快速鉴别
Sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains.
机构信息
Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA.
出版信息
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):12911-6. doi: 10.1073/pnas.1222041110. Epub 2013 Jul 22.
Abstract
Most current diagnostic tests for tuberculosis do not reveal the species or strain of pathogen causing pulmonary infection, which can lead to inappropriate treatment regimens and the spread of disease. Here, we report an assay for mycobacterial strain assignment based on genetically conserved mycobacterial sulfatases. We developed a sulfatase-activated probe, 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one)-sulfate, that detects enzyme activity in native protein gels, allowing the rapid detection of sulfatases in mycobacterial lysates. This assay revealed that mycobacterial strains have distinct sulfatase fingerprints that can be used to judge both the species and lineage. Our results demonstrate the potential of enzyme-activated probes for rapid pathogen discrimination for infectious diseases.
摘要
目前大多数结核病诊断测试都无法揭示引起肺部感染的病原体的种类或菌株，这可能导致治疗方案不当和疾病传播。在这里，我们报告了一种基于遗传上保守的分枝杆菌磺基转移酶的分枝杆菌菌株鉴定检测方法。我们开发了一种磺基转移酶激活探针，7-羟基-9H-(1,3-二氯-9,9-二甲基吖啶-2-酮）-硫酸盐，可在天然蛋白质凝胶中检测酶活性，从而可以快速检测分枝杆菌裂解物中的磺基转移酶。该检测方法显示，分枝杆菌菌株具有独特的磺基转移酶指纹图谱，可用于判断物种和谱系。我们的研究结果表明，酶激活探针具有快速鉴别传染病病原体的潜力。
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