Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
J Colloid Interface Sci. 2023 Oct 15;648:308-316. doi: 10.1016/j.jcis.2023.06.009. Epub 2023 Jun 4.
Biocatalysts are key to the realization of all bioconversions in nature. However, the difficulty of combining the biocatalyst and other chemicals in one system limits their application in artificial reaction systems. Although some effort, such as Pickering interfacial catalysis and enzyme-immobilized microchannel reactors, have addressed this challenge an effective method to combine chemical substrates and biocatalysts in a highly efficient and re-usable monolith system is still to be developed.
A repeated batch-type biphasic interfacial biocatalysis microreactor was developed using enzyme-loaded polymersomes in the void surface of porous monoliths. Polymersomes, loaded with Candida antarctica Lipase B (CALB), are fabricated by self-assembly of the copolymer PEO-b-P(St-co-TMI) and used to stabilize oil-in-water (o/w) Pickering emulsions as a template to prepare monoliths. By adding monomer and Tween 85 to the continuous phase, controllable open-cell monoliths are prepared to inlay CALB-loaded polymersomes in the pore walls.
The microreactor is proven to be highly effective and recyclable when a substrate flows through it, which offers superior benefits of absolute separation to a pure product and no enzyme loss. The relative enzyme activity is constantly maintained above 93% in 15 cycles. The enzyme is constantly present in the microenvironment of the PBS buffer ensuring its immunity to inactivation and facilitating its recycling.
生物催化剂是自然界中所有生物转化实现的关键。然而，将生物催化剂与其他化学物质结合在一个系统中的困难限制了它们在人工反应系统中的应用。尽管已经做出了一些努力，例如 Pickering 界面催化和酶固定化微通道反应器，但仍需要开发一种有效的方法，将化学底物和生物催化剂结合在高效且可重复使用的整体式系统中。
使用多孔整体式中多孔内的聚合物囊负载酶，开发了一种重复批式双相界面生物催化微反应器。聚合物囊通过共聚物 PEO-b-P（St-co-TMI）的自组装制备，并用作水包油（o/w）Pickering 乳液的模板，以制备整体式。通过向连续相中添加单体和 Tween 85，制备可控制的开孔整体式，以将负载 CALB 的聚合物囊镶嵌在孔壁中。
当底物流经微反应器时，证明其具有高效和可重复使用的特点，为纯产物提供了绝对分离的优势，且没有酶的损失。在 15 个循环中，相对酶活性始终保持在 93%以上。酶始终存在于 PBS 缓冲液的微环境中，确保其对失活的免疫性，并促进其回收利用。
