Qiu Penghe, Liu Tong, Xu Yingchun, Ye Chunxiu, Zhang Ran, Wang Yanjie, Jin Qijiang
Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of State Forestry and Grassland Administration on Biology of Ornamental Plants in East China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
College of Forestry and Landscape Architecture, Xinjiang Agricultural University, Urumqi, China.
Hortic Res. 2024 Feb 17;11(6):uhae093. doi: 10.1093/hr/uhae093. eCollection 2024 Jun.
The white water lily (), exemplifying nature's resilience, thrives in the high-altitude terrains of Xinjiang, China, serving as an ideal model for investigating cold adaptation mechanisms in aquatic plants. This study meticulously elucidates the complex cold adaptation mechanisms of the white water lily through a comprehensive and integrated methodological approach. We discovered that the water lily undergoes ecodormancy in winter, retaining high cellular viability and growth potential. During overwintering, the white water lily demonstrates effective resource reallocation, a process facilitated by morphological adjustments, thereby strengthening its resistance to cold temperatures. This enhancement is achieved particularly through the compartmentalization of large vacuoles, the accumulation of osmoregulatory substances, and an increased antioxidant capacity. We established the first exhaustive full-length transcriptome for the white water lily. A subsequent comprehensive analysis of the transcriptome, phytohormones, and metabolome uncovered a multifaceted regulatory network orchestrating cold adaptation. Our research spotlights phytohormone signaling, amino acid metabolism, and circadian rhythms as key elements in the water lily's defense against cold. The results emphasize the critical role of nitrogen metabolism, especially amino acid-related pathways, during cold stress. Metabolite profiling revealed the importance of compounds like myo-inositol and L-proline in enhancing cold tolerance. Remarkably, our study demonstrates that the white water lily notably diminishes the utilization of unsaturated fatty acids in its temperature regulation strategies. In conclusion, this research substantially enriches our understanding of the white water lily's intricate cold adaptation mechanisms, offering new perspectives on the adaptive strategies of aquatic plants and potential applications in agricultural advancement.
白色睡莲（）体现了大自然的适应力，在中国新疆的高海拔地区生长繁茂，是研究水生植物冷适应机制的理想模式植物。本研究通过全面综合的方法，细致阐释了白色睡莲复杂的冷适应机制。我们发现，白色睡莲在冬季进入生态休眠，保持较高的细胞活力和生长潜力。在越冬期间，白色睡莲通过形态调整实现有效的资源重新分配，从而增强其对低温的抗性。这种增强尤其通过大液泡的区室化、渗透调节物质的积累以及抗氧化能力的提高来实现。我们构建了首个白色睡莲详尽的全长转录组。随后对转录组、植物激素和代谢组的综合分析揭示了一个协调冷适应的多方面调控网络。我们的研究突出了植物激素信号传导、氨基酸代谢和昼夜节律在白色睡莲抗寒防御中的关键作用。结果强调了氮代谢，特别是与氨基酸相关的途径在冷胁迫期间的关键作用。代谢物谱分析揭示了肌醇和L-脯氨酸等化合物在增强耐寒性方面的重要性。值得注意的是，我们的研究表明，白色睡莲在其温度调节策略中显著减少了不饱和脂肪酸的利用。总之，本研究极大地丰富了我们对白色睡莲复杂冷适应机制的理解，为水生植物的适应策略及在农业发展中的潜在应用提供了新视角。
