采用宏基因组下一代测序（mNGS）分析血流感染治疗后的细菌 DNA 代谢。
Bacterial DNA metabolism analysis by metagenomic next-generation sequencing (mNGS) after treatment of bloodstream infection.
机构信息
Department of Critical Care Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510080, China.
Department of Critical Care Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510280, China.
出版信息
BMC Infect Dis. 2023 Jun 12;23(1):392. doi: 10.1186/s12879-023-08378-7.
BACKGROUND
With the advent of metagenomic next-generation sequencing (mNGS), the time of DNA metabolism can be explored after bacteria be killed. In this study, we applied mNGS in investigation of the clearance profile of circulating bacteria DNA.
METHODS
All of the rabbits were injected with the inactivated Escherichia coli. Using mNGS, we analyzed serial samples of plasma collected from rabbits to detect clearance profile of circulating E. coli DNA.
RESULTS
In this study, we found that the of E. coli DNA could still be detected 6 h after injecting killed bacteria. The clearance half-lives associated with the 2 phases are 0.37 and 1.81 h. We also explored there is no correlation between the disease severity with the E. coli DNA reads in circulation.
CONCLUSIONS
After the bacteria were completely killed, their DNA could still be detected in the blood circulation. The metabolism of bacterial DNA in the circulation had two phases: fast and slow phases, and there were no correlations between the level of bacteria reads with the severity of patients' disease after the bacteria have been completely killed.
背景
随着宏基因组下一代测序（mNGS）的出现，在细菌被杀死后可以探索 DNA 代谢的时间。在本研究中，我们应用 mNGS 来研究循环细菌 DNA 的清除特征。
方法
所有兔子均注射灭活的大肠杆菌。使用 mNGS 分析从兔子收集的连续血浆样本，以检测循环大肠杆菌 DNA 的清除特征。
结果
在本研究中，我们发现注射灭活细菌后 6 小时仍能检测到大肠杆菌 DNA。与 2 个阶段相关的清除半衰期分别为 0.37 和 1.81 小时。我们还发现，疾病的严重程度与循环中的大肠杆菌 DNA 读取之间没有相关性。
结论
在细菌被完全杀死后，其 DNA 仍能在血液中检测到。循环中细菌 DNA 的代谢有两个阶段：快速和缓慢阶段，并且在细菌完全杀死后，细菌读取的水平与患者疾病的严重程度之间没有相关性。
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