Dong Wanyang, Peng Qi, Tang Xiaohua, Zhong Tian, Lin Shunan, Zhi Ziling, Ye Jingyi, Yang Bixia, Sun Ning, Yuan Wenchang
Guangzhou Key Laboratory for Clinical Rapid Diagnosis and Early Warning of Infectious Diseases, KingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
Third Affiliated Hospital of Guangzhou Medical University, Department of Clinical Laboratory, Guangzhou, 510150, People's Republic of China.
Infect Drug Resist. 2023 Jun 8;16:3639-3647. doi: 10.2147/IDR.S411860. eCollection 2023.
is an opportunistic pathogen that belongs to coagulase-negative (CoNS). Increasing infection and multi-drug resistance cases caused by this strain have been reported and thus it poses a great health threat.
The third-generation sequencing technology was performed on a SH-1 isolated from a clinical sample to analyze the drug resistance genes, which included vancomycin resistance related genes. In addition, antimicrobial susceptibility tests, transmission electron microscopy and Triton X-100 stimulated autolysis were conducted to understand its biological characteristics.
The study shows that this clinical isolate is a vancomycin intermediate-resistant strain. Genome comparison also revealed that WalK(N70K) and WalK(R280Q) mutations may contribute to the vancomycin resistant phenotype. Besides, SH-1 exhibit common features of thicker cell wall and decreased autolytic activity.
SH-1 with WalKR mutations shows typical characteristics of vancomycin resistant strains. Combining the genome features and biological properties, our findings may provide important information for the understanding of the molecular mechanism of to vancomycin intermediate-resistance.
[病原体名称]是一种属于凝固酶阴性葡萄球菌（CoNS）的机会致病菌。据报道，该菌株引起的感染和多重耐药病例不断增加，因此对健康构成了巨大威胁。
对从临床样本中分离出的一株SH-1进行第三代测序技术，以分析耐药基因，其中包括与万古霉素耐药相关的基因。此外，还进行了药敏试验、透射电子显微镜检查和Triton X-100刺激自溶试验，以了解其生物学特性。
研究表明，该临床分离株是一株万古霉素中介耐药菌株。基因组比较还显示，WalK（N70K）和WalK（R280Q）突变可能导致万古霉素耐药表型。此外，SH-1表现出细胞壁较厚和自溶活性降低的共同特征。
具有WalKR突变的SH-1显示出万古霉素耐药菌株的典型特征。结合基因组特征和生物学特性，我们的发现可能为理解[病原体名称]对万古霉素中介耐药的分子机制提供重要信息。
