Feng Shuo, Wu Jianbing, Chen Kunhao, Chen Muxi, Zhu Zhangsheng, Wang Juntao, Chen Guoju, Cao Bihao, Lei Jianjun, Chen Changming
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, 510642 China.
Guangdong Helinong Agricultural Research Institute Co., Ltd, Shantou, 515800 Guangdong China.
Mol Breed. 2023 Mar 3;43(3):17. doi: 10.1007/s11032-023-01364-6. eCollection 2023 Mar.
Mushroom leaves (MLs) are malformed leaves that develop from the leaf veins in some of Chinese kale genotypes. To study the genetic model and molecular mechanism of ML development in Chinese kale, the F segregation population was constructed by two inbred lines, genotype Boc52 with ML and genotype Boc55 with normal leaves (NL). In the present study, we have identified for the first time that the development of mushroom leaves may be affected by the change of adaxial-abaxial polarity of leaves. Examination of the phenotypes of F and F segregation populations suggested that ML development is controlled by two dominant major genes inherited independently. BSA-seq analysis showed that a major quantitative trait locus (QTL) that controls ML development is located within 7.4 Mb on chromosome kC4. The candidate region was further narrowed to 255 kb by linkage analysis combined with insertion/deletion (InDel) markers, and 37 genes were predicted in this region. According to the expression and annotation analysis, a B3 domain-containing transcription factor NGA1-like gene, , was identified as a key candidate gene for controlling ML development in Chinese kale. Fifteen single nucleotide polymorphisms (SNPs) were found in coding sequences and 21 SNPs and 3 InDels found in the promoter sequences of from the genotype Boc52 with ML. The expression levels of in ML genotypes are significantly lower than in the NL genotypes, which suggests that may act as a negative regulator for ML genesis in Chinese kale. This study provides a new foundation for Chinese kale breeding and for the study of the molecular mechanism of plant leaf differentiation.
The online version contains supplementary material available at 10.1007/s11032-023-01364-6.
蘑菇叶（MLs）是一些芥蓝基因型中从叶脉发育而来的畸形叶。为了研究芥蓝中蘑菇叶发育的遗传模式和分子机制，利用两个自交系构建了F分离群体，即具有蘑菇叶的基因型Boc52和具有正常叶（NL）的基因型Boc55。在本研究中，我们首次确定蘑菇叶的发育可能受叶片近轴-远轴极性变化的影响。对F和F分离群体的表型观察表明，蘑菇叶的发育受两个独立遗传的显性主基因控制。全基因组重测序分析表明，一个控制蘑菇叶发育的主要数量性状位点（QTL）位于kC4染色体上7.4 Mb范围内。通过连锁分析结合插入/缺失（InDel）标记，候选区域进一步缩小到255 kb，该区域预测有37个基因。根据表达和注释分析，一个含B3结构域的转录因子NGA1-like基因被确定为控制芥蓝蘑菇叶发育的关键候选基因。在具有蘑菇叶的基因型Boc52的编码序列中发现了15个单核苷酸多态性（SNP），在其启动子序列中发现了21个SNP和3个InDel。该基因在蘑菇叶基因型中的表达水平显著低于正常叶基因型，这表明该基因可能作为芥蓝蘑菇叶发生的负调控因子。本研究为芥蓝育种以及植物叶片分化分子机制的研究提供了新的基础。
在线版本包含可在10.1007/s11032-023-01364-6获取的补充材料。
