Liu Chang, Yang Xiaoxu, He Yongheng, Chen Qifu, Huang Yucheng, Yan Zhishan, Liu Dajun, Feng Guojun
Horticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, 74 Xuefu Road, Harbin, Heilongjiang, 150000 China.
Mol Breed. 2023 Mar 1;43(3):15. doi: 10.1007/s11032-023-01362-8. eCollection 2023 Mar.
Anthocyanin makes snap bean ( L.) pods purple, which helps seed dispersal and protects against environmental stress. In this study, we characterised the snap bean purple mutant , which has purple cotyledon, hypocotyl, stem, leaf vein, flower and pod tissues. Total anthocyanin, delphinidin and malvidin levels in mutant pods were significantly higher than in wild-type plants. We constructed two populations for fine mapping of the purple mutation gene, located in the 243.9-kb region of chromosome 06. We identified , encoding F3'5'H, as a candidate gene for . Six single-base mutations occurred in the coding region of this gene, altering protein structure. and genes were transferred into Arabidopsis, respectively. Compared with the wild-type, the leaf base and internode of T-PV-PUR plant were purple, and the phenotype of T-pv-pur plant remained unchanged, which verified the function of the mutant gene. The results demonstrated that is a crucial gene for anthocyanin biosynthesis in snap bean, resulting in purple colouration. The findings lay a foundation for future breeding and improvement of snap bean.
The online version contains supplementary material available at 10.1007/s11032-023-01362-8.
花青素使菜豆（Phaseolus vulgaris L.）豆荚呈现紫色，这有助于种子传播并抵御环境胁迫。在本研究中，我们对菜豆紫色突变体进行了表征，该突变体的子叶、下胚轴、茎、叶脉、花和豆荚组织均为紫色。突变体豆荚中的总花青素、飞燕草色素和锦葵色素水平显著高于野生型植株。我们构建了两个群体用于精细定位紫色突变基因，该基因位于6号染色体的243.9 kb区域。我们鉴定出编码F3'5'H的PvF3'5'H为紫色突变基因的候选基因。该基因的编码区发生了6个单碱基突变，改变了蛋白质结构。分别将PvF3'5'H和pvf3'5'h基因转入拟南芥。与野生型相比，T-PV-PUR植株的叶基部和节间为紫色，T-pv-pur植株的表型未发生变化，验证了突变基因的功能。结果表明，PvF3'5'H是菜豆花青素生物合成的关键基因，导致了紫色的形成。这些发现为菜豆未来的育种和改良奠定了基础。
在线版本包含可在10.1007/s11032-023-01362-8获取的补充材料。
