菜豆荚中花色苷成分及花色苷生物合成基因的表达分析
Anthocyanin composition and expression analysis of anthocyanin biosynthetic genes in kidney bean pod.
作者信息
Hu Jingtao, Chen Guoping, Zhang Yanjie, Cui Baolu, Yin Wencheng, Yu Xiaohui, Zhu Zhiguo, Hu Zongli
机构信息
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, PR China.
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, PR China.
出版信息
Plant Physiol Biochem. 2015 Dec;97:304-12. doi: 10.1016/j.plaphy.2015.10.019. Epub 2015 Oct 20.
Kidney bean (Phaseolus vulgaris L.) is an important dietary legume crop cultivated and consumed worldwide. A purple cultivar (Zi Bawang) and a green cultivar (Chun Qiu), the main difference of which is in the pod skin color, were selected for the study. Malvidin 3, 5-diglucoside is identified as the major anthocyanin in the pod skin of Zi Bawang by HPLC-ESI-MS/MS. Three regulatory genes PvMYB1, PvMYB2, PvTT8-1 and most structural genes are dramatically up-regulated in purple pod skin compared to those in other materials. Significantly decreased expression levels of all regulatory genes and most biosynthetic genes are also detected in the purple skin of pods covered with bags compared to non-covered ones. All the results suggest that PvMYB1, PvMYB2 and PvTT8-1 might play a critical role in transcriptional activation of most anthocyanin biosynthetic genes in purple kidney bean pod.
菜豆（Phaseolus vulgaris L.）是一种重要的食用豆类作物，在全球范围内种植和消费。本研究选用了一个紫色品种（紫霸王）和一个绿色品种（春秋），它们的主要区别在于豆荚表皮颜色。通过高效液相色谱-电喷雾串联质谱法（HPLC-ESI-MS/MS）鉴定出锦葵色素-3,5-二葡萄糖苷是紫霸王豆荚表皮中的主要花青素。与其他材料相比，三个调控基因PvMYB1、PvMYB2、PvTT8-1以及大多数结构基因在紫色豆荚表皮中显著上调。与未套袋的豆荚相比，套袋豆荚的紫色表皮中所有调控基因和大多数生物合成基因的表达水平也显著降低。所有结果表明，PvMYB1、PvMYB2和PvTT8-1可能在紫色菜豆荚中大多数花青素生物合成基因的转录激活中起关键作用。
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