Du Yanxiu, Liu Lingzhi, Zhang Xiaohua, Li Fei, Kong Fanshu, Zhang Jing, Li Junzhou, Peng Ting, Sun Hongzheng, Zhao Quanzhi
Henan Key Laboratory of Rice Biology, Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou, China.
Mol Breed. 2022 Jun 29;42(7):39. doi: 10.1007/s11032-022-01311-x. eCollection 2022 Jul.
The phytochrome-interacting factor-like gene negatively regulates grain size and 1000-grain weight, but its regulatory effect on rice quality traits is unknown. Here, knock-down, knock-out, and over-expression of transgenic rice lines were used to investigate the effects of on rice yield and quality traits. The results showed that knock-down or knock-out of increased grain length and width, chalkiness, amylose content, glutenin and globulin content, and total protein content but reduced amylopectin content, total starch content, prolamin and albumin content, and gel consistency. Over-expression of showed the opposite results, except for the reduction of prolamin content. Although changed the grain size and weight, it had no effect on grain length/width ratio, brown rice rate, and milled rice rate. KEGG pathway enrichment analysis of differentially expressed genes between transgenic lines and wild type showed that mainly regulated genes related to ribosome, metabolic pathways, and biosynthesis of secondary metabolites. Gene expression analysis showed that RNAi transgenic lines decreased and expression and increased , , , and expression level, while over-expression of increased , , , and and decreased , , and expression level. These results revealed that plays an important role in rice grain development. In addition to grain shape, also regulates chalkiness, starch content, protein content, and gel consistency.
The online version contains supplementary material available at 10.1007/s11032-022-01311-x.
类光敏色素互作因子基因负调控粒型和千粒重，但其对水稻品质性状的调控作用尚不清楚。在此，利用转基因水稻品系的敲低、敲除和过表达来研究其对水稻产量和品质性状的影响。结果表明，敲低或敲除该基因可增加粒长、粒宽、垩白度、直链淀粉含量、谷蛋白和球蛋白含量以及总蛋白含量，但降低支链淀粉含量、总淀粉含量、醇溶蛋白和清蛋白含量以及胶稠度。该基因的过表达则产生相反的结果，但醇溶蛋白含量降低除外。尽管该基因改变了粒型和粒重，但对粒长/粒宽比、糙米率和精米率没有影响。对转基因品系和野生型之间差异表达基因的KEGG通路富集分析表明，该基因主要调控与核糖体、代谢途径和次生代谢物生物合成相关的基因。基因表达分析表明，RNAi转基因品系降低了该基因及其他相关基因的表达，增加了另外一些基因的表达水平，而过表达该基因则增加了部分基因的表达并降低了另外一些基因的表达水平。这些结果表明该基因在水稻籽粒发育中起重要作用。除粒型外，该基因还调控垩白度、淀粉含量、蛋白质含量和胶稠度。
在线版本包含可在10.1007/s11032-022-01311-x获取的补充材料。
