School of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China; Medical Research Center, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.
Department of Stomatology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.
J Plast Surg Hand Surg. 2023 Jun 14;58:26-32. doi: 10.2340/jphs.v58.5775.
Impaired skin wound healing is a common complication of diabetes mellitus. Angiogenesis is a critical step in wound healing because it allows oxygen and nutrients to reach the injured area, thereby promoting wound cell proliferation, re-epithelialisation, and collagen regeneration. However, the neovascularisation ability of patients with diabetes often decreases. Therefore, finding ways to improve diabetic angiogenesis is important for treating diabetic wounds that do not heal. To the best of our knowledge, it is unclear whether dihydroartemisinin (DHA) affects diabetic wounds. This study sought to determine how topical DHA affects the healing of diabetic wounds and how it is related to markers of angiogenesis. We topically applied DHA to full-thickness cutaneous lesions in a streptozotocin (STZ)-induced diabetic mouse model. Under a fluorescence microscope, the pathological morphology of the wound skin was observed, together with the positive expression of platelet endothelial cell adhesion molecule-1 (CD31) and vascular endothelial growth factor (VEGF). Western blotting was used to determine the CD31 and VEGF protein expression levels. The mRNA expression was determined using qualitative real-time polymerase chain reaction (qRT-PCR). We found that DHA can improve the expression of CD31 and VEGF, and accelerate wound healing in diabetic mice. We believe that DHA promotes angiogenesis, which is associated with increased VEGF signalling in vivo. Therefore, DHA can effectively accelerate the process of diabetic wound healing by promoting angiogenesis, implying that DHA may be used as a topical drug for the treatment of diabetic wounds.
皮肤创伤愈合受损是糖尿病的常见并发症。血管生成是创伤愈合的关键步骤，因为它可以使氧气和营养物质到达受伤区域，从而促进创伤细胞增殖、再上皮化和胶原再生。然而，糖尿病患者的新血管生成能力往往会下降。因此，寻找改善糖尿病血管生成的方法对于治疗不愈合的糖尿病伤口非常重要。据我们所知，二氢青蒿素（DHA）是否影响糖尿病伤口尚不清楚。本研究旨在确定局部应用 DHA 如何影响糖尿病伤口的愈合，以及它与血管生成标志物的关系。我们在链脲佐菌素（STZ）诱导的糖尿病小鼠模型中对全层皮肤损伤进行了 DHA 局部应用。在荧光显微镜下，观察了伤口皮肤的病理形态，以及血小板内皮细胞黏附分子-1（CD31）和血管内皮生长因子（VEGF）的阳性表达。使用 Western blot 测定 CD31 和 VEGF 蛋白表达水平。使用定性实时聚合酶链反应（qRT-PCR）测定 mRNA 表达。我们发现 DHA 可以改善 CD31 和 VEGF 的表达，并加速糖尿病小鼠的伤口愈合。我们认为 DHA 通过促进血管生成来促进血管生成，这与体内 VEGF 信号的增加有关。因此，DHA 通过促进血管生成，有效加速糖尿病伤口愈合过程，暗示 DHA 可作为治疗糖尿病伤口的局部药物。
