• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

宏基因组测序揭示了大额牛瘤胃微生物的分类学和功能特征。

Metagenomic Sequencing Reveals the Taxonomic and Functional Characteristics of Rumen Micro-organisms in Gayals.

作者信息

Gao Huan, Yu Ye, Lv Yaqi, Wang Deao, Li Haonan, Li Zhe, Zhang Yuchen, Chen Lan, Leng Jing

机构信息

Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China.

Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.

出版信息

Microorganisms. 2023 Apr 22;11(5):1098. doi: 10.3390/microorganisms11051098.

DOI:10.3390/microorganisms11051098
PMID:37317072
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10223140/
Abstract

As a semi-wild breed, Gayals have a strong fiber degradation capacity, which is unique to the microbial structure and function of their rumen. In this study, the unique rumen microbial composition and function of Gayals were investigated by metagenomic sequencing, with the Yunnan yellow cattle as the control. We compared the differences in rumen micro-organisms between Gayals and the Yunnan Yellow cattle, and the results showed that there were differences in bacteria, archaea and fungi between Gayals and the Yunnan Yellow cattle, while no significant abundance changes were observed in the protozoa. In addition, the ratio of Firmicutes to Bacteroidetes (1.06) in Gayals was higher than that of the Yunnan Yellow cattle (0.66). Three enzymes (PTA, ACH and FTHFS) related to the acetate production pathway and five enzymes (BHBD, THL, PTB, BK and BCACT) involved in butyric acid production were annotated in this study. The CAZymes search results showed that the abundance of GH5, GH26, GH94, CBM11 and CBM63 in Gayals was higher than in the Yunnan Yellow cattle ( < 0.05). Furthermore, this research constructed a model of rumen micro-organisms degrading fibers according to the characteristics and differences in the rumen microbiota structures and functions of the two breeds. This study expands our knowledge of the rumen microbiota and the mechanisms of fiber degradation in Gayals.

摘要

作为半野生品种，大额牛具有很强的纤维降解能力，这与其瘤胃的微生物结构和功能有关。在本研究中，以云南黄牛作为对照，通过宏基因组测序研究了大额牛独特的瘤胃微生物组成和功能。我们比较了大额牛和云南黄牛瘤胃微生物的差异，结果表明，大额牛和云南黄牛在细菌、古菌和真菌方面存在差异，而原生动物的丰度没有显著变化。此外，大额牛中厚壁菌门与拟杆菌门的比例（1.06）高于云南黄牛（0.66）。本研究注释了与乙酸生成途径相关的三种酶（PTA、ACH和FTHFS）以及参与丁酸生成的五种酶（BHBD、THL、PTB、BK和BCACT）。碳水化合物活性酶搜索结果表明，大额牛中GH5、GH26、GH94、CBM11和CBM63的丰度高于云南黄牛（<0.05）。此外，本研究根据两个品种瘤胃微生物群结构和功能的特点及差异，构建了瘤胃微生物降解纤维的模型。本研究扩展了我们对大额牛瘤胃微生物群和纤维降解机制的认识。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/69a3251093d4/microorganisms-11-01098-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/38853f27268c/microorganisms-11-01098-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/c04772656485/microorganisms-11-01098-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/63761fc46c37/microorganisms-11-01098-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/3872e93416ca/microorganisms-11-01098-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/33d2e6e61047/microorganisms-11-01098-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/ee706cb34078/microorganisms-11-01098-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/cce604f36b57/microorganisms-11-01098-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/671c5abeaeb5/microorganisms-11-01098-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/69a3251093d4/microorganisms-11-01098-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/38853f27268c/microorganisms-11-01098-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/c04772656485/microorganisms-11-01098-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/63761fc46c37/microorganisms-11-01098-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/3872e93416ca/microorganisms-11-01098-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/33d2e6e61047/microorganisms-11-01098-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/ee706cb34078/microorganisms-11-01098-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/cce604f36b57/microorganisms-11-01098-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/671c5abeaeb5/microorganisms-11-01098-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d381/10223140/69a3251093d4/microorganisms-11-01098-g009.jpg

相似文献

1
Metagenomic Sequencing Reveals the Taxonomic and Functional Characteristics of Rumen Micro-organisms in Gayals.宏基因组测序揭示了大额牛瘤胃微生物的分类学和功能特征。
Microorganisms. 2023 Apr 22;11(5):1098. doi: 10.3390/microorganisms11051098.
2
The Composition of Fungal Communities in the Rumen of Gayals (), Yaks (), and Yunnan and Tibetan Yellow Cattle ().瘤胃真菌群落组成在羚牛（）、牦牛（）和云南、西藏黄牛（）中的比较。
Pol J Microbiol. 2019 Dec;68(4):505-514. doi: 10.33073/pjm-2019-050. Epub 2019 Dec 5.
3
Dominant bacterial communities in the rumen of Gayals (Bos frontalis), Yaks (Bos grunniens) and Yunnan Yellow Cattle (Bos taurs) revealed by denaturing gradient gel electrophoresis.变性梯度凝胶电泳揭示羚牛（Bos frontalis）、牦牛（Bos grunniens）和云南黄牛（Bos taurs）瘤胃中的优势细菌群落。
Mol Biol Rep. 2011 Nov;38(8):4863-72. doi: 10.1007/s11033-010-0627-8. Epub 2010 Dec 8.
4
Metagenomic analysis exploring taxonomic diversity of rumen microbial communities in Vechur and crossbred cattle of Kerala state, India.印度喀拉拉邦 Vechur 和杂交牛瘤胃微生物群落的分类多样性的宏基因组分析。
J Appl Genet. 2020 May;61(2):287-297. doi: 10.1007/s13353-020-00547-7. Epub 2020 Jan 31.
5
Microbiota composition, gene pool and its expression in Gir cattle (Bos indicus) rumen under different forage diets using metagenomic and metatranscriptomic approaches.利用宏基因组学和宏转录组学方法研究不同饲草日粮下瘤胃中吉拉诺牛（Bos indicus）的微生物群落组成、基因库及其表达。
Syst Appl Microbiol. 2018 Jul;41(4):374-385. doi: 10.1016/j.syapm.2018.02.002. Epub 2018 Mar 9.
6
Understanding the alteration in rumen microbiome and CAZymes profile with diet and host through comparative metagenomic approach.通过比较宏基因组学方法了解饮食和宿主对瘤胃微生物组和 CAZymes 谱的改变。
Arch Microbiol. 2019 Dec;201(10):1385-1397. doi: 10.1007/s00203-019-01706-z. Epub 2019 Jul 23.
7
Metagenomic assessment of the functional potential of the rumen microbiome in Holstein dairy cows.荷斯坦奶牛瘤胃微生物群功能潜力的宏基因组评估
Anaerobe. 2016 Apr;38:50-60. doi: 10.1016/j.anaerobe.2015.12.003. Epub 2015 Dec 15.
8
Diversity and functional analysis of rumen and fecal microbial communities associated with dietary changes in crossbreed dairy cattle.与杂交奶牛日粮变化相关的瘤胃和粪便微生物群落的多样性和功能分析。
PLoS One. 2023 Jan 13;18(1):e0274371. doi: 10.1371/journal.pone.0274371. eCollection 2023.
9
In depth analysis of rumen microbial and carbohydrate-active enzymes profile in Indian crossbred cattle.印度杂交牛瘤胃微生物和碳水化合物活性酶谱的深入分析
Syst Appl Microbiol. 2017 Apr;40(3):160-170. doi: 10.1016/j.syapm.2017.02.003. Epub 2017 Feb 28.
10
Yak rumen microbiome elevates fiber degradation ability and alters rumen fermentation pattern to increase feed efficiency.牦牛瘤胃微生物群提高纤维降解能力并改变瘤胃发酵模式以提高饲料效率。
Anim Nutr. 2022 Aug 9;11:201-214. doi: 10.1016/j.aninu.2022.07.014. eCollection 2022 Dec.

引用本文的文献

1
Environmental Driving of Adaptation Mechanism on Rumen Microorganisms of Sheep Based on Metagenomics and Metabolomics Data Analysis.基于宏基因组学和代谢组学数据分析的绵羊瘤胃微生物适应机制的环境驱动因素。
Int J Mol Sci. 2024 Oct 11;25(20):10957. doi: 10.3390/ijms252010957.
2
Analysis of Rumen Degradation Characteristics, Attached Microbial Community, and Cellulase Activity Changes of Garlic Skin and Stalk.大蒜皮和蒜秸的瘤胃降解特性、附着微生物群落及纤维素酶活性变化分析
Animals (Basel). 2024 Jan 4;14(1):169. doi: 10.3390/ani14010169.

本文引用的文献

1
An integrated gene catalog and over 10,000 metagenome-assembled genomes from the gastrointestinal microbiome of ruminants.反刍动物胃肠道微生物组的综合基因目录和超过 10000 个宏基因组组装基因组。
Microbiome. 2021 Jun 12;9(1):137. doi: 10.1186/s40168-021-01078-x.
2
Metagenomic analysis exploring taxonomic diversity of rumen microbial communities in Vechur and crossbred cattle of Kerala state, India.印度喀拉拉邦 Vechur 和杂交牛瘤胃微生物群落的分类多样性的宏基因组分析。
J Appl Genet. 2020 May;61(2):287-297. doi: 10.1007/s13353-020-00547-7. Epub 2020 Jan 31.
3
Effects of live yeast on differential genetic and functional attributes of rumen microbiota in beef cattle.
活酵母对肉牛瘤胃微生物群差异遗传和功能特性的影响。
J Anim Sci Biotechnol. 2019 Sep 4;10:68. doi: 10.1186/s40104-019-0378-x. eCollection 2019.
4
Comparing the Microbial Community in Four Stomach of Dairy Cattle, Yellow Cattle and Three Yak Herds in Qinghai-Tibetan Plateau.青藏高原奶牛、黄牛四个胃以及三个牦牛群微生物群落的比较
Front Microbiol. 2019 Jul 10;10:1547. doi: 10.3389/fmicb.2019.01547. eCollection 2019.
5
Ruminal microbiome-host crosstalk stimulates the development of the ruminal epithelium in a lamb model.瘤胃微生物组-宿主串扰刺激羔羊模型中瘤胃上皮的发育。
Microbiome. 2019 Jun 3;7(1):83. doi: 10.1186/s40168-019-0701-y.
6
Comparative metagenomic and metatranscriptomic analyses reveal the breed effect on the rumen microbiome and its associations with feed efficiency in beef cattle.比较宏基因组学和宏转录组学分析揭示了品种对瘤胃微生物组的影响及其与肉牛饲料效率的关系。
Microbiome. 2019 Jan 14;7(1):6. doi: 10.1186/s40168-019-0618-5.
7
A metagenomic analysis of the camel rumen's microbiome identifies the major microbes responsible for lignocellulose degradation and fermentation.对骆驼瘤胃微生物组的宏基因组分析确定了负责木质纤维素降解和发酵的主要微生物。
Biotechnol Biofuels. 2018 Aug 2;11:216. doi: 10.1186/s13068-018-1214-9. eCollection 2018.
8
Metagenomic binning reveals the functional roles of core abundant microorganisms in twelve full-scale biogas plants.宏基因组binning 揭示了十二座全规模沼气厂中核心优势微生物的功能作用。
Water Res. 2018 Sep 1;140:123-134. doi: 10.1016/j.watres.2018.04.043. Epub 2018 Apr 20.
9
Microbiota composition, gene pool and its expression in Gir cattle (Bos indicus) rumen under different forage diets using metagenomic and metatranscriptomic approaches.利用宏基因组学和宏转录组学方法研究不同饲草日粮下瘤胃中吉拉诺牛（Bos indicus）的微生物群落组成、基因库及其表达。
Syst Appl Microbiol. 2018 Jul;41(4):374-385. doi: 10.1016/j.syapm.2018.02.002. Epub 2018 Mar 9.
10
Thermophilic xylanases: from bench to bottle.嗜热木聚糖酶：从实验室到生产。
Crit Rev Biotechnol. 2018 Nov;38(7):989-1002. doi: 10.1080/07388551.2018.1425662. Epub 2018 Jan 17.