Martín-González Diego, Bordel Sergio, Santos-Beneit Fernando
Institute of Sustainable Processes, Dr. Mergelina, s/n, 47011 Valladolid, Spain.
Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain.
Microorganisms. 2023 Apr 24;11(5):1109. doi: 10.3390/microorganisms11051109.
In this study, we describe the characterization of three efficient chicken feather-degrading bacteria isolated from honeybee samples and assess the impact of their co-cultivation on this activity and antistaphylococcal activity. AD2 was the strain showing the highest keratinolytic activity (4000 U × mL), followed by AN1 and AD1, which both generated approximately 3000 U × mL. Moreover, a consortium constituted of these three strains was able to use chicken feathers as its sole nutrient source and growth in such conditions led to a significant increase in antibiotic production. AD2 was the only strain that exhibited weak antimicrobial activity against . UPLC analyses revealed that a significant number of peaks detected in the extracts of co-cultures of the three strains were missing in the extracts of individual cultures. In addition, the production of specialized metabolites, such as undecylprodigiosin and manumycin A, was clearly enhanced in co-culture conditions, in agreement with the results of the antimicrobial bioassays against . Our results revealed the benefits of co-cultivation of these bacterial species in terms of metabolic wealth and antibiotic production. Our work could thus contribute to the development of novel microbial-based strategies to valorize keratin waste.
在本研究中，我们描述了从蜜蜂样本中分离出的三种高效降解鸡毛细菌的特性，并评估了它们共培养对这种活性和抗葡萄球菌活性的影响。AD2是表现出最高角蛋白分解活性（4000 U×mL）的菌株，其次是AN1和AD1，它们的活性均约为3000 U×mL。此外，由这三种菌株组成的联合体能够将鸡毛作为其唯一营养源，在这种条件下生长导致抗生素产量显著增加。AD2是唯一对……表现出微弱抗菌活性的菌株。超高效液相色谱分析表明，在三种菌株共培养物提取物中检测到的大量峰在单个培养物提取物中缺失。此外，在共培养条件下，诸如灵菌红素和马奴霉素A等特殊代谢产物的产量明显提高，这与针对……的抗菌生物测定结果一致。我们的结果揭示了这些细菌物种共培养在代谢丰富度和抗生素生产方面的益处。因此，我们的工作有助于开发基于微生物的新型策略，以实现角蛋白废物的增值利用。
