Institute of Sustainable Processes, Dr. Mergelina S/N, 47011, Valladolid, Spain.
Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Dr. Mergelina, S/N, 47011, Valladolid, Spain.
Mol Genet Genomics. 2023 Mar;298(2):389-398. doi: 10.1007/s00438-022-01989-w. Epub 2022 Dec 31.
Keratin-rich wastes, mainly in the form of feathers, are recalcitrant residues generated in high amounts as by-products in chicken farms and food industry. Polylactic acid (PLA) is the second most common biodegradable polymer found in commercial plastics, which is not easily degraded by microbial activity. This work reports the 3.8-Mb genome of Bacillus altitudinis B12, a highly efficient PLA- and keratin-degrading bacterium, with potential for environmental friendly biotechnological applications in the feed, fertilizer, detergent, leather, and pharmaceutical industries. The whole genome sequence of B. altitudinis B12 revealed that this strain (which had been previously misclassified as Bacillus pumilus B12) is closely related to the B. altitudinis strains ER5, W3, and GR-8. A total of 4056 coding sequences were annotated using the RAST server, of which 2484 are core genes of the pan genome of B. altitudinis and 171 are unique to this strain. According to the sequence analysis, B. pumilus B12 has a predicted secretome of 353 proteins, among which a keratinase and a PLA depolymerase were identified by sequence analysis. The presence of these two enzymes could explain the characterized PLA and keratin biodegradation capability of the strain.
富含角蛋白的废物主要以羽毛的形式存在，是鸡场和食品工业大量产生的难以生物降解的副产物。聚乳酸（PLA）是商业塑料中第二常见的可生物降解聚合物，不易被微生物活性降解。本工作报道了高度有效降解 PLA 和角蛋白的芽孢杆菌 B12 的 3.8Mb 基因组，该菌具有在饲料、肥料、洗涤剂、皮革和制药等行业进行环保生物技术应用的潜力。芽孢杆菌 B12 的全基因组序列表明，该菌株（先前被错误分类为短小芽孢杆菌 B12）与芽孢杆菌 ER5、W3 和 GR-8 菌株密切相关。使用 RAST 服务器共注释了 4056 个编码序列，其中 2484 个是芽孢杆菌泛基因组的核心基因，171 个是该菌株所特有的。根据序列分析，短小芽孢杆菌 B12 具有预测的 353 种蛋白质分泌组，其中通过序列分析鉴定出了角蛋白酶和 PLA 解聚酶。这两种酶的存在可以解释该菌株对 PLA 和角蛋白的生物降解能力。
