El Aila Nabil Abdullah, Al Laham Nahed Ali, Naas Thierry
Department of Medical Laboratory Sciences, Faculty of Medical Sciences, Al-Aqsa University Gaza, Gaza P.O. Box 405, Palestine.
Department of Laboratory Medicine, Faculty of Medical Sciences, Al-Azhar University-Gaza, Gaza P.O. Box 1277, Palestine.
Microorganisms. 2023 Apr 28;11(5):1155. doi: 10.3390/microorganisms11051155.
Methicillin-resistant (MRSA) are spreading worldwide in hospital and community settings, thus posing a serious public health problem. Panton-Valentine Leukocidin (PVL), an important virulence factor of , is a marker of community-acquired MRSA. Here we determined the prevalence of genes among isolates from different hospitals in the Gaza Strip, Palestine. A total of 285 isolates were collected from five different hospitals in the Gaza Strip. All isolates were characterized for their susceptibility patterns to available antimicrobial agents and by using multiplex PCR for the detection of and genes. The overall prevalence of MRSA in Gaza hospitals was 70.2% (range: 76.3% to 65.5%) and that of among isolates was 29.8% (range: 32.9% to 26.2%). The gene was equally prevalent among MRSA isolates (30.5%) and MSSA isolates (28.2%). The most effective antibiotics were rifampicin, vancomycin, and clindamycin, with susceptibility rates of 91.2%, 88.7%, and 84.6%, respectively. The highest percentage of strains were observed to be resistant to penicillin and amoxicillin with clavulanic acid-96.1% and 73.6%, respectively. Our results showed a high prevalence of MRSA and -positive isolates in Gaza Strip hospitals, which likely reflects the situation in the community. It is mandatory to implement systematic surveillance of both hospital and community isolates, together with interventions (such as increased hand hygiene, use of hydroalcoholic solutions, and isolation of carriers) to limit their spread.
耐甲氧西林金黄色葡萄球菌（MRSA）正在医院和社区环境中全球传播，从而构成严重的公共卫生问题。杀白细胞素（PVL）是MRSA的一种重要毒力因子，是社区获得性MRSA的一个标志。在此，我们确定了巴勒斯坦加沙地带不同医院的285株金黄色葡萄球菌分离株中PVL基因的流行情况。所有分离株均通过对现有抗菌药物的敏感性模式进行表征，并使用多重PCR检测PVL和mecA基因。加沙医院中MRSA的总体流行率为70.2%（范围：76.3%至65.5%），PVL在金黄色葡萄球菌分离株中的流行率为29.8%（范围：32.9%至26.2%）。PVL基因在MRSA分离株（30.5%）和甲氧西林敏感金黄色葡萄球菌（MSSA）分离株（28.2%）中同样普遍。最有效的抗生素是利福平、万古霉素和克林霉素，敏感性率分别为91.2%、88.7%和84.6%。观察到最高比例的菌株对青霉素和阿莫西林/克拉维酸耐药，分别为96.1%和73.6%。我们的结果显示加沙地带医院中MRSA和PVL阳性分离株的高流行率，这可能反映了社区的情况。必须对医院和社区分离株进行系统监测，并采取干预措施（如加强手卫生、使用含酒精的溶液和隔离携带者）以限制其传播。
