Rodriguez-Yupanqui Magda, De La Cruz-Noriega Magaly, Quiñones Claudio, Otiniano Nélida Milly, Quezada-Alvarez Medardo A, Rojas-Villacorta Walter, Vergara-Medina Gino A, León-Vargas Frank R, Solís-Muñoz Haniel, Rojas-Flores Segundo
Escuela de Ingeniería Ambiental, Facultad de Ingeniería y Arquitectura, Universidad Cesar Vallejo, Trujillo 13007, Peru.
Instituto de Investigación en Ciencia y Tecnología, Universidad César Vallejo, Trujillo 13001, Peru.
Microorganisms. 2023 Apr 29;11(5):1168. doi: 10.3390/microorganisms11051168.
The effluents generated in the paper industry, such as black liquor, have a high content of lignin and other toxic components; however, they represent a source of lignin-degrading bacteria with biotechnological potential. Therefore, the present study aimed to isolate and identify lignin-degrading bacteria species in paper mill sludge. A primary isolation was carried out from samples of sludge present in environments around a paper company located in the province of Ascope (Peru). Bacteria selection was made by the degradation of Lignin Kraft as the only carbon source in a solid medium. Finally, the laccase activity (Um-L) of each selected bacteria was determined by oxidation of 2,2'-azinobis-(3-etilbencenotiazolina-6-sulfonate) (ABTS). Bacterial species with laccase activity were identified by molecular biology techniques. Seven species of bacteria with laccase activity and the ability to degrade lignin were identified. The bacteria (2), (1), and (1) were reported for first time. and presented the highest laccase activity, with values of 0.319 ± 0.005 UmL and 0.329 ± 0.004 UmL, respectively. In conclusion, paper mill sludge may represent a source of lignin-degrading bacteria with laccase activity, and they could have potential biotechnological applications.
造纸工业产生的废水，如黑液，含有高含量的木质素和其他有毒成分；然而，它们是具有生物技术潜力的木质素降解细菌的来源。因此，本研究旨在分离和鉴定造纸厂污泥中的木质素降解细菌种类。从位于阿斯科佩省（秘鲁）一家造纸公司周围环境中的污泥样本进行初步分离。通过在固体培养基中以木质素硫酸盐作为唯一碳源进行细菌筛选。最后，通过氧化2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）来测定每种选定细菌的漆酶活性（Um-L）。通过分子生物学技术鉴定具有漆酶活性的细菌种类。鉴定出7种具有漆酶活性和降解木质素能力的细菌。细菌（2）、（1）和（1）首次被报道。和呈现出最高的漆酶活性，分别为0.319±0.005 UmL和0.329±0.004 UmL。总之，造纸厂污泥可能是具有漆酶活性的木质素降解细菌的来源，并且它们可能具有潜在的生物技术应用。
