Division of Microbiology and Parasitology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.
Unit of Excellence in Research and Product Development of Coffee, Division of Physiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.
J Microbiol Biotechnol. 2023 Sep 28;33(9):1179-1188. doi: 10.4014/jmb.2304.04051. Epub 2023 May 24.
, particularly multidrug-resistant (MDR) strains, is a serious cause of healthcare-associated infections. Development of novel antimicrobial agents or restoration of drug efficiency is required to treat MDR bacteria, and the use of natural products to solve this problem is promising. We investigated the antimicrobial activity of dried green coffee (DGC) beans, coffee pulp (CP), and arabica leaf (AL) crude extracts against 28 isolated MDR strains and restoration of ampicillin (AMP) efficiency with a combination test. DGC, CP, and AL extracts were effective against all 28 strains, with a minimum inhibitory concentration (MIC) of 12.5-50 mg/ml and minimum bactericidal concentration of 25-100 mg/ml. The CP-AMP combination was more effective than CP or AMP alone, with a fractional inhibitory concentration index value of 0.01. In the combination, the MIC of CP was 0.2 mg/ml (compared to 25 mg/ml of CP alone) and that of AMP was 0.1 mg/ml (compared to 50 mg/ml of AMP alone), or a 125-fold and 500-fold reduction, respectively, against 13-drug resistant MDR strains. Time-kill kinetics showed that the bactericidal effect of the CP-AMP combination occurred within 3 h through disruption of membrane permeability and biofilm eradication, as verified by scanning electron microscopy. This is the first report indicating that CP-AMP combination therapy could be employed to treat MDR by repurposing AMP.
，尤其是多药耐药（MDR）菌株，是导致与医疗保健相关的感染的严重原因。需要开发新型抗菌剂或恢复药物效率来治疗 MDR 细菌，利用天然产物来解决这个问题是有前途的。我们研究了干绿咖啡豆（DGC）、咖啡果肉（CP）和阿拉比卡叶（AL）粗提取物对 28 株分离的 MDR 菌株的抗菌活性，并通过组合试验研究了恢复氨苄青霉素（AMP）效率的情况。DGC、CP 和 AL 提取物对所有 28 株菌株均有效，最低抑菌浓度（MIC）为 12.5-50mg/ml，最低杀菌浓度为 25-100mg/ml。CP-AMP 联合用药比 CP 或 AMP 单独用药更有效，部分抑菌浓度指数值为 0.01。在联合用药中，CP 的 MIC 为 0.2mg/ml（与 CP 单独使用的 25mg/ml 相比），AMP 的 MIC 为 0.1mg/ml（与 AMP 单独使用的 50mg/ml 相比），分别降低了 125 倍和 500 倍，对 13 种耐药 MDR 菌株有效。时间杀伤动力学表明，CP-AMP 联合用药通过破坏细胞膜通透性和生物膜清除，在 3 小时内即可发挥杀菌作用，这通过扫描电子显微镜得到了验证。这是第一项表明可以通过重新利用 AMP 来治疗 MDR 的 CP-AMP 联合治疗的报告。
