School of Sport Sciences, Nanjing Normal University, Nanjing, China.
School of Sport Sciences, Nanjing Normal University, Nanjing, China.
Biochem Biophys Res Commun. 2023 Sep 3;671:292-300. doi: 10.1016/j.bbrc.2023.06.027. Epub 2023 Jun 7.
Aging adipose tissue exhibits elevated inflammation and oxidative stress that are major sources of age-related metabolic dysfunction. However, the exact metabolic changes associated with inflammation and oxidative stress are unclear. To address this topic, we assessed variation in metabolic phenotypes of adipose tissue from 18 months adult sedentary (ASED), 26 months old sedentary (OSED), and 8 months young sedentary (YSED). The results of metabolomic analysis showed that ASED and OSED group had higher palmitic acid, elaidic acid, 1-heptadecanol, and α-tocopherol levels than YSED, but lower sarcosine levels. Furthermore, stearic acid was specifically elevated in ASED compared with YSED. Cholesterol was upregulated specifically in the OSED group compared with YSED, whereas linoleic acid was downregulated. In addition, ASED and OSED had more inflammatory cytokines, lower antioxidant capacity, and higher expression of ferroptosis-related genes than YSED. Moreover, mitochondrial dysfunction associated with abnormal cardiolipin synthesis was more pronounced in the OSED group. In conclusion, both ASED and OSED can affect the FA metabolism and increase oxidative stress in adipose tissue, leading to inflammation. In particular, linoleic acid content specifically decreases in OSED, which associated with abnormal cardiolipin synthesis and mitochondrial dysfunction in adipose tissue.
衰老的脂肪组织表现出炎症和氧化应激的升高，这是与年龄相关的代谢功能障碍的主要来源。然而，与炎症和氧化应激相关的确切代谢变化尚不清楚。为了解决这个问题，我们评估了来自 18 个月大的久坐不动的成年人（ASED）、26 个月大的久坐不动的成年人（OSED）和 8 个月大的久坐不动的年轻人（YSED）的脂肪组织代谢表型的变化。代谢组学分析的结果表明，ASED 和 OSED 组的棕榈酸、反式油酸、1-十七烷醇和α-生育酚水平高于 YSED，但肌氨酸水平较低。此外，与 YSED 相比，ASED 中硬脂酸的水平特别升高。与 YSED 相比，胆固醇在 OSED 组中特异性地上调，而亚油酸则下调。此外，与 YSED 相比，ASED 和 OSED 具有更多的炎症细胞因子、更低的抗氧化能力和更高的铁死亡相关基因表达。此外，与异常心磷脂合成相关的线粒体功能障碍在 OSED 组中更为明显。总之，ASED 和 OSED 都会影响脂肪组织中的 FA 代谢并增加氧化应激，导致炎症。特别是在 OSED 中，亚油酸的含量特异性降低，这与脂肪组织中异常的心磷脂合成和线粒体功能障碍有关。
