Yu Rui, Xiong Zhiying, Zhu Xinhui, Feng Panpan, Hu Ziyi, Fang Rongxiang, Zhang Yuman, Liu Qinglin
Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, China.
National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, and National Plant Gene Research Center, Beijing 100101, China.
Hortic Res. 2023 Apr 25;10(6):uhad083. doi: 10.1093/hr/uhad083. eCollection 2023 Jun.
Rose (), which is an economically valuable floral species worldwide, has three types, namely once-flowering (OF), occasional or re-blooming (OR), and recurrent or continuous flowering (CF). However, the mechanism underlying the effect of the age pathway on the duration of the CF or OF juvenile phase is largely unknown. In this study, we observed that the transcript levels were substantially upregulated during the floral development period in CF and OF plants. Additionally, accumulation of RcSPL1 protein was controlled by rch-miR156. The ectopic expression of in accelerated the vegetative phase transition and flowering. Furthermore, the transient overexpression of in rose plants accelerated flowering, whereas silencing of had the opposite phenotype. Accordingly, the transcription levels of floral meristem identity genes (, , and ) were significantly affected by the changes in expression. RcTAF15b protein, which is an autonomous pathway protein, was revealed to interact with RcSPL1. The silencing and overexpression of in rose plants led to delayed and accelerated flowering, respectively. Collectively, the study findings imply that RcSPL1-RcTAF15b modulates the flowering time of rose plants.
玫瑰（蔷薇属）是全球具有经济价值的花卉物种，有三种类型，即一次开花型（OF）、偶发或再次开花型（OR）和反复或连续开花型（CF）。然而，年龄途径对CF或OF幼年期持续时间影响的潜在机制在很大程度上尚不清楚。在本研究中，我们观察到在CF和OF植物的花期发育阶段，转录水平显著上调。此外，RcSPL1蛋白的积累受rch-miR156调控。在拟南芥中异位表达加速了营养生长阶段的转变和开花。此外，在玫瑰植株中瞬时过表达加速了开花，而沉默则产生相反的表型。因此，花分生组织特征基因（、和）的转录水平受表达变化的显著影响。RcTAF15b蛋白是自主途径蛋白，被发现与RcSPL1相互作用。在玫瑰植株中沉默和过表达分别导致开花延迟和加速。总体而言，研究结果表明RcSPL1-RcTAF15b调节玫瑰植株的开花时间。
