Tang Yanping, Gao Ruotian, Ma Changle, Wang Jin, Li Jing
College of Biological Science and Food Engineering, Southwest Forestry University, Kunming, Yunnan, China.
Forest Resources Exploitation and Utilization Engineering Research Center for Grand Health of Yunnan Provincial Universities, Southwest Forestry University, Kunming, Yunnan, China.
Front Microbiol. 2025 Jun 4;16:1598458. doi: 10.3389/fmicb.2025.1598458. eCollection 2025.
Chinese rose powdery mildew, caused by , is a devastating disease which has a significant impact on plants' ornamental and economic value. Strain KMR13, which exhibited pronounced mycoparasitic activity against , was isolated during the initial phase of this study; however, the underlying mechanism remains to be elucidated.
In order to analyze the biological control and mycoparasitism mechanisms, the present study was carried out to sequence the whole genome of strain KMR13 using a combination of second-generation Illumina and third-generation nanopore platforms, to mine chitinase genes from the KMR13 genome, and to screen for chitinase genes related to mycoparasitism by detecting the expression of the genes at different time periods of sporulation induction.
The results revealed a genome size of 33,532,117 base pairs (bp) with a GC content of 50.97%, encoding 12,545 genes and 379 non-coding RNAs. Functional annotations using NR, GO, KOG, Pfam, and KEGG databases identified 12,355, 8,208, 1,871, 7,911, and 7,657 genes, respectively. A total of 15 GH18 family genes were mined in KMR13, and a total of 10 chitinase genes were detected to be expressed in the transcriptome under spore induction, 5 genes were consistently up-regulated for expression after induction, and 5 genes had the highest expression at 24h of induction. RT-qPCR analysis of 5 genes with high expression as well as high fold expression showed significant differential expression for all genes, with the highest expression at 24 h of induction. Up-regulated expression of after induction is likely to play a role in disrupting the spore wall during mycoparasitic process of strain KMR13. Field trials demonstrated that KMR13 conidial suspensions significantly suppressed -induced powdery mildew, although the control efficacy was lower than that observed under greenhouse conditions.
These findings collectively highlight the potential of KMR13 as a biological control agent and provide a theoretical foundation for eco-friendly management of Chinese rose powdery mildew.
由[病原菌名称缺失]引起的月季白粉病是一种毁灭性病害，对植物的观赏价值和经济价值有重大影响。在本研究的初始阶段分离出了对[病原菌名称缺失]表现出显著重寄生活性的KMR13菌株；然而，其潜在机制仍有待阐明。
为了分析生物防治和重寄生机制，本研究采用二代Illumina和三代纳米孔平台相结合的方法对KMR13菌株的全基因组进行测序，从KMR13基因组中挖掘几丁质酶基因，并通过检测在孢子形成诱导的不同时间段基因的表达来筛选与重寄生相关的几丁质酶基因。
结果显示基因组大小为33,532,117碱基对（bp），GC含量为50.97%，编码12,545个基因和379个非编码RNA。使用NR、GO、KOG、Pfam和KEGG数据库进行功能注释分别鉴定出12,355、8,208、1,871、7,911和7,657个基因。在KMR13中共挖掘到15个GH18家族基因，在转录组中检测到共有10个几丁质酶基因在孢子诱导下表达，5个基因在诱导后持续上调表达，5个基因在诱导24小时时表达量最高。对5个高表达以及高倍数表达的基因进行RT-qPCR分析，结果显示所有基因均有显著差异表达，在诱导24小时时表达量最高。诱导后[基因名称缺失]的上调表达可能在KMR13菌株的重寄生过程中破坏孢子壁发挥作用。田间试验表明，KMR13分生孢子悬浮液显著抑制了[病原菌名称缺失]引起的白粉病，尽管防治效果低于温室条件下观察到的效果。
这些发现共同突出了KMR13作为生物防治剂的潜力，并为月季白粉病的生态友好型管理提供了理论基础。
