Cimmino Carlos, Rodrigues Capítulo Leandro, Lerman Andrea, Silva Andrea, Von Haften Gabriela, Comino Ana P, Cigoy Luciana, Scagliola Marcelo, Poncet Verónica, Caló Gonzalo, Uez Osvaldo, Berón Corina M
Instituto Nacional de Epidemiología "Dr. Juan H. Jara" Mar del Plata Argentina Instituto Nacional de Epidemiología "Dr. Juan H. Jara", Mar del Plata, Argentina.
Centro de Estudios Integrales de la Dinámica Exógena Universidad Nacional de La Plata La Plata Argentina Centro de Estudios Integrales de la Dinámica Exógena, Universidad Nacional de La Plata, La Plata, Argentina.
Rev Panam Salud Publica. 2023 Jun 12;47:e94. doi: 10.26633/RPSP.2023.94. eCollection 2023.
To implement and evaluate the use of wastewater sampling for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in two coastal districts of Buenos Aires Province, Argentina.
In General Pueyrredon district, 400 mL of wastewater samples were taken with an automatic sampler for 24 hours, while in Pinamar district, 20 L in total (2.2 L at 20-minute intervals) were taken. Samples were collected once a week. The samples were concentrated based on flocculation using polyaluminum chloride. RNA purification and target gene amplification and detection were performed using reverse transcription polymerase chain reaction for clinical diagnosis of human nasopharyngeal swabs.
In both districts, the presence of SARS-CoV-2 was detected in wastewater. In General Pueyrredon, SARS-CoV-2 was detected in epidemiological week 28, 2020, which was 20 days before the start of an increase in coronavirus virus disease 2019 (COVID-19) cases in the first wave (epidemiological week 31) and 9 weeks before the maximum number of laboratory-confirmed COVID-19 cases was recorded. In Pinamar district, the virus genome was detected in epidemiological week 51, 2020 but it was not possible to carry out the sampling again until epidemiological week 4, 2022, when viral circulation was again detected.
It was possible to detect SARS-CoV-2 virus genome in wastewater, demonstrating the usefulness of the application of wastewater epidemiology for long-term SARS-CoV-2 detection and monitoring.
在阿根廷布宜诺斯艾利斯省的两个沿海地区实施并评估利用废水采样检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的情况。
在普埃雷东将军区，使用自动采样器采集24小时的废水样本，每次400毫升；而在皮纳马尔区，共采集20升（每隔20分钟采集2.2升）。每周采集一次样本。样本通过使用聚氯化铝进行絮凝浓缩。采用逆转录聚合酶链反应进行RNA纯化以及靶基因扩增和检测，用于人类鼻咽拭子的临床诊断。
在两个地区的废水中均检测到了SARS-CoV-2。在普埃雷东将军区，于2020年第28个流行病学周检测到SARS-CoV-2，这比第一波冠状病毒病2019（COVID-19）病例增加开始前20天（第31个流行病学周），以及实验室确诊COVID-19病例数达到峰值前9周。在皮纳马尔区，于2020年第51个流行病学周检测到病毒基因组，但直到2022年第4个流行病学周才再次进行采样，此时再次检测到病毒传播。
能够在废水中检测到SARS-CoV-2病毒基因组，证明了废水流行病学应用于长期SARS-CoV-2检测和监测的有效性。
