Scarpini E, Meola G, Baron P, Beretta S, Velicogna M, Scarlato G
Exp Neurol. 1986 Jul;93(1):77-83. doi: 10.1016/0014-4886(86)90146-9.
Human fetal and adult Schwann cells, which had been maintained in culture for 5 weeks according to an explant-reexplantation technique, were labeled by immunoperoxidase using antibodies directed against S-100 protein and laminin in order to find specific antigenic markers. Immunocytochemical analysis of the distribution of both proteins showed that they were expressed in long-term cultures. The localization of S-100 protein and laminin in long-term cultures indicated that the expression of these proteins by human Schwann cells was not axon-dependent and also occurred in absence of myelin synthesis.
根据外植体-再植技术在培养中维持5周的人胎儿和成人雪旺细胞，使用针对S-100蛋白和层粘连蛋白的抗体通过免疫过氧化物酶进行标记，以寻找特异性抗原标志物。对这两种蛋白质分布的免疫细胞化学分析表明，它们在长期培养物中表达。S-100蛋白和层粘连蛋白在长期培养物中的定位表明，人雪旺细胞对这些蛋白质的表达不依赖于轴突，并且在没有髓鞘合成的情况下也会发生。
