Heimfeld S, Bode H R
Dev Biol. 1986 Jul;116(1):51-8. doi: 10.1016/0012-1606(86)90042-4.
The interstitial cells of hydra contain a stem cell population which produces several classes of differentiated cell types. A model has been proposed which governs the growth rate of the interstitial cell population. This model, based on the density of interstitial cells in the tissue, makes specific predictions about the relationships among this density, the proportion of stem cells in the interstitial cell population, the growth rate of the interstitial cell population, and the amount of nematocyte differentiation. Hydroxyurea treatments were used to experimentally reduce interstitial cell numbers, and the validity of these expected correlations was tested. The results demonstrate that the predictions of the interstitial cell density model were not upheld. Furthermore, the findings suggest that the interstitial cells are a heterogeneous population, containing some cells which are no longer stem cells but which do retain a limited capacity for proliferation. In the following paper (S. Heimfeld and H.R. Bode, 1986, Dev. Biol. 115, 59-68) we have proposed an alternative mechanism to explain the observed correlations, which incorporates this heterogeneity into amplification divisions of interstitial cells already committed to differentiation.
水螅的间质细胞包含一个干细胞群体，该群体可产生几类分化的细胞类型。已提出一个模型来控制间质细胞群体的生长速率。这个基于组织中间质细胞密度的模型，对该密度、间质细胞群体中干细胞的比例、间质细胞群体的生长速率以及刺细胞分化量之间的关系做出了具体预测。使用羟基脲处理来实验性地减少间质细胞数量，并检验这些预期相关性的有效性。结果表明，间质细胞密度模型的预测未得到支持。此外，研究结果表明间质细胞是一个异质群体，包含一些不再是干细胞但仍保留有限增殖能力的细胞。在接下来的论文（S. Heimfeld和H.R. Bode，1986年，《发育生物学》115卷，59 - 68页）中，我们提出了一种替代机制来解释观察到的相关性，该机制将这种异质性纳入已承诺分化的间质细胞的扩增分裂中。
