Littlefield C L
Dev Biol. 1985 Nov;112(1):185-93. doi: 10.1016/0012-1606(85)90132-0.
Single clones of interstitial cells were generated and analyzed to determine if one interstitial cell has the capacity to differentiate both somatic and germ cells. Such clones were produced by using hydroxyurea to selectively eliminate interstitial cells from normal Hydra oligactis males. The number of animals devoid of interstitial cells within the population was determined by staining whole animals with toluidine blue which renders the interstitial cells visible. The number of animals containing single clones of interstitial cells was then estimated using single hit Poisson statistics. In treatments which rendered 60-80% of the population devoid of interstitial cells, the majority of the animals containing interstitial cells lost the ability to produce somatic cells, including nerves and nematocytes, but retained the capacity to produce sperm. This result strongly suggests the presence of a separate germ line in hydra.
生成并分析了间质细胞的单个克隆，以确定单个间质细胞是否有能力分化出体细胞和生殖细胞。通过使用羟基脲从正常的寡柄水螅雄性个体中选择性地消除间质细胞来产生这样的克隆。通过用甲苯胺蓝对整个动物进行染色来确定群体中不含间质细胞的动物数量，甲苯胺蓝可使间质细胞可见。然后使用单次打击泊松统计来估计含有间质细胞单个克隆的动物数量。在使60 - 80%的群体不含间质细胞的处理中，大多数含有间质细胞的动物失去了产生包括神经细胞和刺细胞在内的体细胞的能力，但保留了产生精子的能力。这一结果有力地表明水螅中存在独立的生殖系。
