Hadi Soltanabad Mojtaba, Bagherieh-Najjar Mohammad B, Mianabadi Manijeh
Department of Biology, Golestan University, Shahid Beheshti Ave., Gorgan, IR, Iran.
AryaTinaGene Bio-pharmaceutical Company, Gorgan, IR, Iran.
Appl Biochem Biotechnol. 2020 Jun;191(2):482-495. doi: 10.1007/s12010-019-03193-w. Epub 2019 Dec 4.
Biosynthesis of carnosic acid (CA), one of the most industrially valuable medicinal compounds present in rosemary (Rosmarinus officinalis L.) leaves, is affected by various plant stressors. In this study, effects of silver nanoparticle (AgNP) treatment on the secondary metabolism and CA production of rosemary plants were investigated. AgNP of 0, 25, 50, 100, and 200 ppm were utilized on hydroponically grown plants using foliar spray. Efficient absorbance and translocation of AgNPs to the plant roots were confirmed by XRF (X-ray fluorescence) analysis. The fluctuations of important antioxidant compounds such as CA content, phenolics, flavonoids, and acid ascorbic were analyzed and their correlations evaluated. Results revealed that application of 200 ppm AgNPs for 12 days increased CA level more than 11%, as compared to the control plants. Furthermore, significant positive correlations were observed between total flavonoids and CA content under AgNP treatment, suggesting that AgNP acted as an elicitor and triggered the enhancement of CA accumulation effectively. These data suggest that concentration-dependent AgNP may be used to boost antioxidant activity and phytochemical contents of other medicinal plants.
迷迭香叶中存在的工业上最有价值的药用化合物之一——鼠尾草酸（CA）的生物合成，受到各种植物应激源的影响。在本研究中，研究了银纳米颗粒（AgNP）处理对迷迭香植物次生代谢和CA产生的影响。使用叶面喷施法，将0、25、50、100和200 ppm的AgNP施用于水培植物。通过X射线荧光（XRF）分析证实了AgNP能有效吸收并转运到植物根部。分析了重要抗氧化化合物如CA含量、酚类、黄酮类和抗坏血酸的波动情况，并评估了它们之间的相关性。结果表明，与对照植物相比，施用200 ppm AgNP 12天可使CA水平提高超过11%。此外，在AgNP处理下，总黄酮与CA含量之间存在显著正相关，表明AgNP作为一种激发子，有效触发了CA积累的增强。这些数据表明，浓度依赖性的AgNP可用于提高其他药用植物的抗氧化活性和植物化学成分含量。
