Department of Gastrointestinal Surgery, The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, 225300, Jiangsu, China.
Oncology department, The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, 225300, Jiangsu, China.
BMC Cancer. 2023 Jun 17;23(1):559. doi: 10.1186/s12885-023-11047-2.
Peritoneal metastasis is one of the main causes of death in patients with gastric cancer (GC). Galectin-1 regulates various undesirable biological behaviors in GC and may be key in GC peritoneal metastasis.
In this study, we elucidated the regulatory role of galectin-1 in GC cell peritoneal metastasis. GC and peritoneal tissues underwent hematoxylin-eosin (HE), immunohistochemical (IHC), and Masson trichrome staining to analyze the difference in galectin-1 expression and peritoneal collagen deposition in different GC clinical stages. The regulatory role of galectin-1 in GC cell adhesion to mesenchymal cells and in collagen expression was determined using HMrSV5 human peritoneal mesothelial cells (HPMCs). Collagen and corresponding mRNA expression were detected with western blotting and reverse transcription PCR, respectively. The promoting effect of galectin-1 on GC peritoneal metastasis was verified in vivo. Collagen deposition and collagen I, collagen III, and fibronectin 1 (FN1) expression in the peritoneum of the animal models were detected by Masson trichrome and IHC staining.
Galectin-1 and collagen deposition in the peritoneal tissues was correlated with GC clinical staging and were positively correlated. Galectin-1 enhanced the ability of GC cells to adhere to the HMrSV5 cells by promoting collagen I, collagen III, and FN1 expression. The in vivo experiments confirmed that galectin-1 promoted GC peritoneal metastasis by promoting peritoneal collagen deposition.
Galectin-1-induced peritoneal fibrosis may create a favorable environment for GC cell peritoneal metastasis.
腹膜转移是胃癌（GC）患者死亡的主要原因之一。半乳糖凝集素-1（Galectin-1）调控 GC 中的各种不良生物学行为，可能是 GC 腹膜转移的关键。
本研究阐明了 Galectin-1 在 GC 细胞腹膜转移中的调控作用。对 GC 和腹膜组织进行苏木精-伊红（HE）、免疫组织化学（IHC）和 Masson 三色染色，以分析不同 GC 临床分期中 Galectin-1 表达和腹膜胶原沉积的差异。用人腹膜间皮细胞（HPMCs）HMrSV5 检测 Galectin-1 对 GC 细胞与间质细胞黏附以及胶原表达的调控作用。通过 Western blot 和逆转录 PCR 分别检测胶原和相应的 mRNA 表达。在体内验证 Galectin-1 对 GC 腹膜转移的促进作用。通过 Masson 三色和 IHC 染色检测动物模型中腹膜的胶原沉积以及胶原 I、胶原 III 和纤维连接蛋白 1（FN1）的表达。
Galectin-1 和腹膜组织中的胶原沉积与 GC 临床分期相关，呈正相关。Galectin-1 通过促进胶原 I、胶原 III 和 FN1 表达增强 GC 细胞与 HMrSV5 细胞的黏附能力。体内实验证实 Galectin-1 通过促进腹膜胶原沉积促进 GC 腹膜转移。
Galectin-1 诱导的腹膜纤维化可能为 GC 细胞腹膜转移创造有利环境。
