Barbatis C, Morton J, Woods J C, Burns J, Bradley J, McGee J O
Gut. 1986 Jul;27(7):765-70. doi: 10.1136/gut.27.7.765.
The distribution of Mallory body antigens JMB1 and 2 was examined in 82 human fresh diagnostic needle liver biopsies and 28 necropsies by the indirect immunoperoxidase technique using 2 monoclonal antibodies (anti-JMB1 and 2) against Mallory bodies. The JMB1 antigen was detectable in bile duct epithelium and in hepatocytes of histologically normal livers. It was also found in all Mallory bodies in various hepatic disorders. This antigen was markedly increased in the cytoplasm of all liver cells in acute alcoholic hepatitis superimposed on alcoholic cirrhosis, in most cases of acute alcoholic hepatitis, and in severe fatty infiltration of the liver with or without Mallory body formation. Mallory bodies contained this antigen but the cytoplasm of Mallory body containing cells lacked JMB1. In normal liver the JMB2 antigen was localised on the cytoplasmic intermediate filament network of hepatocytes and bile duct epithelium; and almost all Mallory bodies also contained this antigen but the adjacent cytoplasm of these cells lacked JMB2. In severe alcoholic liver disease these antigens could not be detected in large zones of hepatocytes even when these hepatocytes did not contain Mallory bodies. It is evident that there is disorganisation of intermediate filament constituents in severe alcoholic liver disease.
采用两种针对马洛里小体的单克隆抗体（抗JMB1和抗JMB2），运用间接免疫过氧化物酶技术，在82例新鲜的人类诊断性肝脏穿刺活检标本和28例尸检标本中检测马洛里小体抗原JMB1和JMB2的分布情况。在组织学正常肝脏的胆管上皮细胞和肝细胞中可检测到JMB1抗原。在各种肝脏疾病的所有马洛里小体中也能发现该抗原。在酒精性肝硬化基础上并发的急性酒精性肝炎的所有肝细胞胞质中、大多数急性酒精性肝炎病例以及伴有或不伴有马洛里小体形成的严重肝脏脂肪浸润中，该抗原均显著增加。马洛里小体含有此抗原，但含马洛里小体的细胞胞质中缺乏JMB1。在正常肝脏中，JMB2抗原定位于肝细胞和胆管上皮细胞的胞质中间丝网络；几乎所有的马洛里小体也含有此抗原，但这些细胞相邻的胞质中缺乏JMB2。在严重酒精性肝病中，即使肝细胞不含马洛里小体，在大片肝细胞区域也检测不到这些抗原。显然，在严重酒精性肝病中存在中间丝成分的紊乱。
