miR-361-5p 作为颈动脉狭窄的生物标志物，促进血管平滑肌细胞增殖和迁移。
MicroRNA-361-5p acts as a biomarker for carotid artery stenosis and promotes vascular smooth muscle cell proliferation and migration.
机构信息
Department of Neurosurgery, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwen District, 261035, Weifang, Shandong, China.
Department of Image Center, Affiliated Hospital of Weifang Medical University, 261035, Weifang, China.
出版信息
BMC Med Genomics. 2023 Jun 16;16(1):134. doi: 10.1186/s12920-023-01563-2.
BACKGROUND
Vascular smooth muscle cells (VSMCs) dysfunction participates in carotid artery stenosis (CAS). The study aimed to examine the expression pattern of miR-361-5p in CAS patients, and explore its role in VSMCs proliferation and migration.
METHODS
qRT-PCR was performed for the detection of miR-361-5p in serum samples of 150 CAS cases and 150 healthy people. Multiple logistic regression analysis and receiver operating characteristic (ROC) curve was accomplished to detect diagnostic value via SPSS 21.0 statistical software. Cell function of VSMCs was evaluated. Target association was predicted through bioinformatic analysis and confirmed via luciferase activity.
RESULTS
Serum miR-361-5p was enhanced in CAS cases and was positively correlated with CAS degree. Logistic regression analysis determined the independent influence of miR-361-5p in CAS, and ROC curve demonstrated its diagnostic value with AUC of 0.892. miR-361-5p promoted VSMCs proliferation and migration, but the influence was counteracted by TIMP4.
CONCLUSIONS
MiR-361-5p is a promising biomarker for CAS, and can be used as a potential target for early diagnosis and treatment of CAS. MiR-361-5p can promote VSMCs proliferation and migration via targeting TIMP4.
背景
血管平滑肌细胞（VSMCs）功能障碍参与颈动脉狭窄（CAS）。本研究旨在检测 miR-361-5p 在 CAS 患者中的表达模式，并探讨其在 VSMCs 增殖和迁移中的作用。
方法
通过 qRT-PCR 检测 150 例 CAS 患者和 150 例健康人的血清样本中的 miR-361-5p。通过 SPSS 21.0 统计软件完成多因素逻辑回归分析和受试者工作特征（ROC）曲线，以检测诊断价值。评估 VSMCs 的细胞功能。通过生物信息学分析预测靶标关联，并通过荧光素酶活性进行验证。
结果
CAS 患者血清 miR-361-5p 升高，且与 CAS 程度呈正相关。逻辑回归分析确定了 miR-361-5p 在 CAS 中的独立影响，ROC 曲线显示其诊断价值，AUC 为 0.892。miR-361-5p 促进 VSMCs 的增殖和迁移，但 TIMP4 可拮抗其影响。
结论
miR-361-5p 是 CAS 的一种有前途的生物标志物，可作为 CAS 早期诊断和治疗的潜在靶点。miR-361-5p 通过靶向 TIMP4 促进 VSMCs 的增殖和迁移。
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