College of Landscape Architecture, Zhejiang A&F University, Hangzhou, 311300, China; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China.
Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, 51006, Estonia.
Plant Physiol Biochem. 2023 Aug;201:107841. doi: 10.1016/j.plaphy.2023.107841. Epub 2023 Jun 14.
Drought stress occurs more frequently in recent years due to the global climate change. Widely distributed in northern China, Mongolia, and Russia, Trollius chinensis Bunge has high medicinal and ornamental values and is often exposed to drought stress, while the mechanism underlying its drought response is still unclear. In this study, we applied 74-76% (control, CK), 49-51% (mild drought), 34-36% (moderate drought), and 19-21% (severe drought, SD) of the soil gravimetric water content to T. chinensis, and measured leaf physiological characteristics on the 0, 5th, 10th, 15th day after the soil reaching the set drought severities, and on the 10th day after rehydration. The results showed that many physiological parameters, such as chlorophyll contents, Fv/Fm, ΦPSⅡ, P, and g decreased with the deepening of severity and duration of drought stress and recovered to some extent after rehydration. On the 10th day of drought stress, leaves in SD and CK were selected for RNA-Seq, and 1649 differentially expressed genes (DEGs) were found, including 548 up-regulated and 1101 down-regulated DEGs. Gene Ontology enrichment found that the DEGs were mainly enriched in catalytic activity and thylakoid. Koyto Encyclopedia of Genes and Genomes enrichment found that DEGs were enriched in some metabolic pathways such as carbon fixation and photosynthesis. Among them, the differential expression of genes related to photosynthesis process, ABA biosynthesis and signaling pathway, such as NCED, SnRK2, PsaD, PsbQ, and PetE, might explain why T. chinensis could tolerate and recover from as long as 15 days of severe drought conditions.
由于全球气候变化，近年来干旱胁迫更为频繁。紫菀广泛分布于中国北方、蒙古和俄罗斯，具有很高的药用和观赏价值，经常受到干旱胁迫的影响，但它对干旱的响应机制尚不清楚。在这项研究中，我们采用了 74-76%（对照，CK）、49-51%（轻度干旱）、34-36%（中度干旱）和 19-21%（重度干旱，SD）的土壤重量含水量来处理紫菀，并在土壤达到设定干旱严重程度后的第 0、5、10、15 天以及复水后的第 10 天测量叶片生理特性。结果表明，许多生理参数，如叶绿素含量、Fv/Fm、ΦPSⅡ、P 和 g，随着干旱胁迫严重程度和持续时间的加深而降低，并在复水后有所恢复。在干旱胁迫的第 10 天，我们从 SD 和 CK 叶片中选择了 RNA-Seq，发现了 1649 个差异表达基因（DEGs），包括 548 个上调和 1101 个下调的 DEGs。基因本体论富集发现，DEGs 主要富集在催化活性和类囊体中。京都基因与基因组百科全书富集发现，DEGs 富集在一些代谢途径中，如碳固定和光合作用。其中，与光合作用过程、ABA 生物合成和信号通路相关的基因如 NCED、SnRK2、PsaD、PsbQ 和 PetE 的差异表达，可能解释了为什么紫菀能够耐受长达 15 天的重度干旱条件并从中恢复。
