研究报告：从美国野生火鸡粪便中回收的艾美耳球虫属的分离、种型鉴定和抗球虫药敏感性。

Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States.

机构信息

Department of Poultry Science, University of Arkansas Division of Agriculture, Fayetteville AR, 72701, USA.

Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Ciudad de México 04510, México.

出版信息

Poult Sci. 2023 Aug;102(8):102819. doi: 10.1016/j.psj.2023.102819. Epub 2023 May 29.

DOI:10.1016/j.psj.2023.102819

PMID:37331218

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10404749/

Abstract

Between 2018 and 2020, over 100 wild turkey fecal samples were collected from the Eastern and Central thirds of the United States, where commercial turkey production is uncommon. We hypothesized that anticoccidial-sensitive Eimeria spp. would be present in wild turkey fecal samples. Samples containing Eimeria spp. oocysts were amplified in vivo. If propagation was successful, the samples were PCR-speciated and subjected to anticoccidial sensitivity testing (AST) for key members of both ionophore and chemical categories of anticoccidial drugs. The purpose of this study was to isolate Eimeria spp. relevant to commercial turkey production that possessed sensitivity to monensin, zoalene, and amprolium. Future research would evaluate the efficacy of wild turkey Eimeria spp. as vaccine candidates for reducing coccidiosis in commercial turkey flocks utilizing single oocyst-derived stocks obtained in the present study.

摘要

在 2018 年至 2020 年期间，从美国东部和中部地区（商业火鸡生产不常见）采集了 100 多份野生火鸡粪便样本。我们假设在野生火鸡粪便样本中存在对抗球虫药物敏感的艾美尔球虫。含有艾美尔球虫卵囊的样本在体内进行扩增。如果繁殖成功，样本将通过 PCR 进行分类，并对离子载体和化学类抗球虫药物的关键成员进行抗球虫药物敏感性测试（AST）。本研究的目的是分离与商业火鸡生产相关且对莫能菌素、马杜霉素和常山酮敏感的艾美尔球虫。未来的研究将评估利用本研究中获得的单卵囊衍生株作为疫苗候选物来减少商业火鸡群球虫病的野生火鸡艾美尔球虫的功效。