Zoology Department, Faculty of Science, Damanhur University, Egypt.
Zoology Department, Faculty of Science, Damanhur University, Egypt.
Tissue Cell. 2023 Aug;83:102127. doi: 10.1016/j.tice.2023.102127. Epub 2023 Jun 9.
It is well known that diabetes is associated with impairment of ovarian and testicular structure and function. Coriander (Coriandrum sativum L.) is identified as one of the oldest herbal plants valued for its nutritional and medicinal properties. This work is mainly designed to evaluate the possible modulatory role of dry coriander fruit extract against gonadal impairments associated with diabetes in female rats and their pups. Twenty-four pregnant rats were divided into four groups (n = 6): group I served as control, group II was administered daily with coriander fruit extract (250 mg/kg b.wt), group III was injected interaperitoneally with a single dose of streptozotocin (STZ) (80 mg/kg b.wt), and group IV was injected with single dose of STZ and post administered coriander extract. The experiment was conducted from the 4th day of gestation till the end of weaning. At the end of the experiment, the mothers' rats and their offspring were weighed, sacrificed, the ovaries from mothers as well as ovaries and testes from offspring were immediately excised, and processed for histological, immunohistochemical and evaluation of apoptosis and transforming growth factorβ (TGF-β). Also, blood samples were collected and analyzed to estimate the levels of sex hormones as well as antioxidants.In STZ induced diabetes in mother's rats and their offspring, the ovarian sections revealed severe histopathological signs included several atretic follicles, dilated and congested blood capillaries. Additionally, the testicular sections of offspring appeared with destructive seminiferous tubules. Immunohistochemically, the ovarian sections displayed weak to negative expression for calretinin marker however the testicular sections showed strong expression for Bax protein (apoptotic marker) and weak to negative expression for Ki67 protein (proliferative marker). Also, the mean % values of positively expressed cells for TGF-β and annexin-v markers (late and early apoptosis indicator) were significantly elevated in the ovarian and testicular tissues of STZ-induced group of mother's rats and their pups if compared with control. Further results revealed that the levels of insulin, FSH, LH, estrogen, SOD and CAT were significantly decreased if compared with control however the levels of MDA and NO were significantly elevated. Administration of coriander fruit extract to diabetic rats successfully alleviated most of the altered histological, immunohistochemical, biochemical, and apoptotic changes induced by diabetes. Coriandrum sativum fruit extract has a powerful ameliorative role against STZ-induced diabetic gonadal dysfunctions in female rats and their offspring.
众所周知，糖尿病与卵巢和睾丸结构和功能的损伤有关。芫荽（Coriandrum sativum L.）被认为是最古老的草药植物之一，具有营养价值和药用价值。这项工作主要旨在评估干芫荽果提取物对糖尿病雌性大鼠及其幼崽性腺损伤的可能调节作用。24 只怀孕大鼠被分为四组（n=6）：第 I 组为对照组，第 II 组每天给予芫荽果提取物（250mg/kg 体重），第 III 组腹腔注射链脲佐菌素（STZ）（80mg/kg 体重），第 IV 组注射 STZ 后给予芫荽提取物。实验从妊娠第 4 天进行到断奶结束。实验结束时，母鼠及其幼崽称重，处死，立即取出母鼠的卵巢以及幼崽的卵巢和睾丸，并进行组织学、免疫组织化学以及凋亡和转化生长因子β（TGF-β）的评估。此外，采集血液样本并进行分析以估计性激素和抗氧化剂的水平。在 STZ 诱导的母鼠及其幼崽糖尿病中，卵巢切片显示严重的组织病理学特征，包括多个闭锁卵泡和扩张充血的毛细血管。此外，幼崽的睾丸切片显示破坏性的曲细精管。免疫组织化学显示，钙调蛋白标志物在卵巢切片中的表达较弱或为阴性，而 Bax 蛋白（凋亡标志物）在睾丸切片中的表达较强，Ki67 蛋白（增殖标志物）的表达较弱或为阴性。此外，与对照组相比，STZ 诱导组母鼠及其幼崽的卵巢和睾丸组织中 TGF-β和膜联蛋白-V 标志物（晚期和早期凋亡标志物）的阳性细胞的平均%值显著升高。进一步的结果表明，与对照组相比，胰岛素、FSH、LH、雌激素、SOD 和 CAT 的水平显著降低，而 MDA 和 NO 的水平显著升高。芫荽果提取物对糖尿病大鼠的给药成功缓解了糖尿病引起的大多数组织学、免疫组织化学、生化和凋亡变化。芫荽果提取物对 STZ 诱导的雌性大鼠及其幼崽糖尿病性腺功能障碍具有强大的改善作用。
