College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, China; School of Forestry, Northeast Forestry University, Harbin 150040, China.
School of Forestry, Northeast Forestry University, Harbin 150040, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2023 Sep;271:109687. doi: 10.1016/j.cbpc.2023.109687. Epub 2023 Jun 17.
Pine wilt disease (PWD), caused by Bursaphelenchus xylophilus (pine wood nematodes, PWNs), is a forest disease that seriously threatens the health of Pinus forestry. Glutathione S-transferases (GSTs) play important roles in xenobiotic metabolism, lipophilic compound transport, antioxidative stress reactions, anti-mutagenesis, and antitumor activity. The analysis and investigation of the specific functions of GSTs in the metabolism of toxic substances in nematodes are important for identifying potential target genes to control the spread and transmission of B. xylophilus. In this study, 51 Bx-GSTs were found in the genome of B. xylophilus. Two key Bx-gsts (Bx-gst12 and Bx-gst40) were analyzed when B. xylophilus was exposed to avermectin. The expression of Bx-gst12 and Bx-gst40 was significantly increased when B. xylophilus was exposed to 1.6 and 3.0 mg/mL avermectin solutions. Notably, combined silencing of both Bx-gst12 and Bx-gst40 did not further increase the mortality rates under avermectin exposure. Mortality rates were significantly increased in nematodes treated with dsRNA compared to control nematodes (p < 0.05) after RNAi. The feeding ability of nematodes was also significantly reduced after treatment with dsRNA. These results suggested that Bx-gsts are associated with the detoxification process and feeding behavior of B. xylophilus. Silencing Bx-gsts leads to increased susceptibility to nematicides and reduces the feeding ability of B. xylophilus. Therefore, Bx-gsts will be a new control target of PWNs in the future.
松材线虫病（PWD）是由松材线虫（PWNs）引起的一种森林病害，严重威胁着松林的健康。谷胱甘肽 S-转移酶（GSTs）在异生物质代谢、脂溶性化合物转运、抗氧化应激反应、抗突变和抗肿瘤活性中发挥重要作用。分析和研究 GSTs 在线虫体内有毒物质代谢中的特定功能，对于鉴定潜在的靶基因以控制松材线虫的传播和传播具有重要意义。在本研究中，在松材线虫基因组中发现了 51 个 Bx-GSTs。当松材线虫暴露于阿维菌素时，分析了两个关键的 Bx-gsts（Bx-gst12 和 Bx-gst40）。当松材线虫暴露于 1.6 和 3.0 mg/mL 的阿维菌素溶液时，Bx-gst12 和 Bx-gst40 的表达明显增加。值得注意的是，当同时沉默 Bx-gst12 和 Bx-gst40 时，在阿维菌素暴露下死亡率并没有进一步增加。与对照线虫相比，用 dsRNA 处理的线虫死亡率在 RNAi 后显著增加（p < 0.05）。用 dsRNA 处理后，线虫的取食能力也显著降低。这些结果表明，Bx-GSTs 与松材线虫的解毒过程和取食行为有关。沉默 Bx-GSTs 会导致对杀线虫剂的敏感性增加，并降低松材线虫的取食能力。因此，Bx-GSTs 将成为未来松材线虫的一个新的控制靶点。
