Thaumatin-Like Protein-1 基因（）与的致病性有关。

Thaumatin-Like Protein-1 Gene () Is Associated with the Pathogenicity of .

机构信息

Laboratory of Forest Pathogen Integrated Biology, Research Institute of Forestry New Technology, Chinese Academy of Forestry, Beijing, P.R. China.

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing Jiangsu, P.R. China.

出版信息

Phytopathology. 2019 Nov;109(11):1949-1956. doi: 10.1094/PHYTO-03-19-0082-R. Epub 2019 Sep 30.

DOI:10.1094/PHYTO-03-19-0082-R

PMID:31573422

Abstract

The pine wood nematode is a destructive species affecting pine trees worldwide; however, the underlying mechanism leading to pathogenesis remains unclear. In this study, a gene encoding thaumatin-like protein-1 () was silenced by RNA interference to clarify the relationship between the gene and pathogenicity. The in vitro knockdown of with double-stranded RNA (dsRNA) decreased reproduction and pathogenicity. Treatments with dsRNA targeting decreased expression by 90%, with the silencing effect maintained even in the F3 offspring. Pine trees inoculated with treated with dsRNA decreased the symptom of wilting, and the disease severity index was 56.7 at 30 days after inoculation. Additionally, analyses of the cavitation of intact pine stem samples by X-ray microtomography revealed that the xylem cavitation area of pine trees inoculated with treated with dsRNA was 0.46 mm at 30 days after inoculation. Results from this study indicated that the silencing of has effects on fitness. The data presented here provide the foundation for future analyses of functions related to pathogenicity.

摘要

松材线虫是一种危害全世界松树的破坏性物种；然而，导致发病的潜在机制仍不清楚。在这项研究中，通过 RNA 干扰沉默了一个编码 Thaumatin-like protein-1 ()的基因，以阐明该基因与致病性之间的关系。用双链 RNA (dsRNA)体外敲低会降低的繁殖和致病性。针对的 dsRNA 处理使表达降低了 90%，即使在 F3 后代中也能保持沉默效果。用 dsRNA 处理的感染的松树萎蔫症状减轻，接种后 30 天的病情严重指数为 56.7。此外，通过 X 射线微断层扫描对完整松茎样本的空化分析表明，接种后 30 天用 dsRNA 处理的感染的松树木质部空化面积为 0.46mm。本研究结果表明，沉默对的适应性有影响。这里提供的数据为未来分析与致病性相关的功能奠定了基础。