The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083 P.R. China.
Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, P.R. China.
Phytopathology. 2022 Apr;112(4):888-897. doi: 10.1094/PHYTO-07-21-0289-R. Epub 2022 Mar 21.
Pine wilt disease is a major forest disease worldwide, including in China, where it has severely damaged pine forest ecosystems, and the pathogen is pine wood nematode (). The thaumatin-like protein-1 gene (--) is a key gene associated with . pathogenicity, which is also responsive to α-pinene. In this study, an examination of seedlings infected by . revealed that monoterpene (sesquiterpene) levels peaked on days 15 and 27 (days 18 and 27). Meanwhile, . - expression levels were high on days 3, 12, and 27, which were consistent with the expression of key enzymes genes in the terpene biosynthesis pathway. The functional similarity of . Bx-TLP-1 and . Pm-TLP suggests Bx-TLP-1 and Pm-TLP may have similar roles in . . There was also no secondary accumulation of terpenes in . seedlings during . treated with dsRNA targeting -- (dsTLP1) infections, reflecting the decreased pathogenicity of . and the delayed disease progression in pine trees. And the results of micro-CT showed that the degree of cavitation for the trees inoculated with Bx-TLP-1 (0.3811 mm) was greater than that for the trees inoculated with dsTLP1 PWNs (0.1204 mm) on day 15 after inoculation. Results from this study indicated that . -- gene may induce the upregulated expression of related genes encoding enzymes in the terpene synthesis pathway of . , resulting in the accumulation of terpenes, which also provided an insight to investigate the . pathogenicity in the future.
松材线虫病是一种全球性的重要森林病害，包括在中国，该病已严重破坏了松林生态系统，其病原体是松材线虫（）。苏氨酸蛋白酶样蛋白-1 基因（--）是与致病性相关的关键基因，同时也对α-蒎烯有响应。在本研究中，对感染松材线虫（）的幼苗进行了检测，发现单萜（倍半萜）水平在第 15 天和第 27 天（第 18 天和第 27 天）达到峰值。同时，--表达水平在第 3、12 和 27 天较高，这与萜烯生物合成途径中的关键酶基因的表达一致。Bx-TLP-1 和 Pm-TLP 的功能相似，表明 Bx-TLP-1 和 Pm-TLP 在松材线虫中可能具有相似的作用。在 dsRNA 靶向--（dsTLP1）感染的幼苗中，也没有萜类物质的二次积累，这反映了松材线虫的致病性降低和松树病情进展的延迟。微 CT 的结果表明，在接种 Bx-TLP-1 的树木中（0.3811mm）的空蚀程度大于在接种 dsTLP1 PWNs 的树木中（0.1204mm）的空蚀程度，这是在接种后第 15 天。本研究结果表明，--基因可能诱导与萜烯合成途径相关的基因上调表达，导致萜烯的积累，这为未来研究松材线虫的致病性提供了思路。
