Unit of Toxicology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.
Unit of Toxicology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.
Comp Biochem Physiol C Toxicol Pharmacol. 2023 Sep;271:109688. doi: 10.1016/j.cbpc.2023.109688. Epub 2023 Jun 17.
Octocrylene (OC), an UV-filter (OUVF) is used in many cosmetic products to protect the skin against the harmful effects of UV radiation. Octocrylene has been detected in the environment and become an emerging contaminant of concern. However, the eco-toxicological data on octocrylene and their molecular effects and mechanism of action on freshwater fish are very limited. In this research work, the potential toxicity of octocrylene and its mechanisms on morphology, antioxidant and AChE activity, apoptosis, and histopathological changes were investigated in embryonic zebrafish (Danio rerio) at different concentrations (5, 50 and 500 μg/L). Embryos/larvae (96 hpf) treated with 50 and 500 μg/L of OC caused developmental abnormalities, and decreased hatching rate and heartbeat rate. The oxidative damage (LPO) and antioxidant enzyme (SOD, CAT and GST) activities were apparently elevated (P < 0.05) at the highest test concentration (500 μg/L). However, acetylcholinesterase (AChE) activity was significantly inhibited at the highest test concentration. Also, OC induced apoptosis in a dose-dependent manner. The zebrafish exposed to 50 and 500 μg/L showed histopathological changes including elongated yolk sac, swim bladder inflammation, muscle cell degeneration, retinal damage and pyknotic cells. In conclusion, octocrylene has induced oxidative stress at environmentally relevant concentrations leading to developmental toxicity, neurotoxicity, and histopathological damage in zebrafish embryos/larvae.
辛氧苯酮（OC）是一种紫外线过滤剂（OUVF），广泛用于许多化妆品中，以保护皮肤免受紫外线辐射的有害影响。辛氧苯酮已在环境中被检测到，成为一种新兴的关注污染物。然而，关于辛氧苯酮的生态毒理学数据及其对淡水鱼类的分子作用机制和作用机制非常有限。在这项研究工作中，不同浓度（5、50 和 500μg/L）的辛氧苯酮对胚胎斑马鱼（Danio rerio）的形态、抗氧化和 AChE 活性、细胞凋亡和组织病理学变化的潜在毒性及其机制进行了研究。用 50 和 500μg/L 的 OC 处理的胚胎/幼虫（96 hpf）导致发育异常，并降低孵化率和心率。在最高测试浓度（500μg/L）下，氧化损伤（LPO）和抗氧化酶（SOD、CAT 和 GST）活性明显升高（P<0.05）。然而，乙酰胆碱酯酶（AChE）活性在最高测试浓度下显著受到抑制。此外，OC 以剂量依赖的方式诱导细胞凋亡。暴露于 50 和 500μg/L 的斑马鱼表现出组织病理学变化，包括卵黄囊伸长、鳔炎、肌肉细胞退化、视网膜损伤和固缩细胞。总之，辛氧苯酮在环境相关浓度下诱导氧化应激，导致斑马鱼胚胎/幼虫发育毒性、神经毒性和组织病理学损伤。
