Laboratory of Animal Reproduction, Graduate School of Veterinary Sciences, Azabu University, Sagamihara 252-5201, Japan.
School of Veterinary Medicine, Azabu University, Sagamihara 252-5201, Japan.
J Reprod Dev. 2023 Aug 11;69(4):223-226. doi: 10.1262/jrd.2023-036. Epub 2023 Jun 17.
Superovulation procedures are routinely and widely used in mouse reproductive technology. Previous studies have shown that a large number of oocytes can be obtained from adult mice (> 10 weeks old) using a combined treatment with progesterone (P4) and anti-inhibin serum (AIS). However, these effects have not been fully investigated in young (4 weeks) C57BL/6J mice. Here, we found that a modified superovulation protocol (combined treatment with P4, AIS, eCG (equine chorionic gonadotropin), and hCG (human chorionic gonadotropin); P4D2-Ae-h) improved the number of oocytes compared to the control (eCG and hCG) (39.7 vs. 21.3 oocytes/mouse). After in vitro fertilization, pronuclear formation rates were 69.3% (P4D2-Ae-h group) and 66.2% (control group). After embryo transfer, 46.4% (116/250) of the embryos in the P4D2-Ae-h group successfully developed to term, which was comparable to the control group (42.9%; 123/287 embryos). In conclusion, our protocol (P4D2-Ae-h) was effective for superovulation in young C57BL/6J mice.
超数排卵程序在小鼠生殖技术中被常规广泛应用。先前的研究表明，通过使用孕激素（P4）和抗抑制素血清（AIS）联合处理，可以从成年小鼠（>10 周龄）中获得大量卵母细胞。然而，这些效果在年轻（4 周龄）C57BL/6J 小鼠中尚未得到充分研究。在这里，我们发现改良的超数排卵方案（联合使用 P4、AIS、eCG（马绒毛膜促性腺激素）和 hCG（人绒毛膜促性腺激素）；P4D2-Ae-h）与对照组（eCG 和 hCG）相比，可提高卵母细胞数量（39.7 比 21.3 个/只）。体外受精后，原核形成率分别为 69.3%（P4D2-Ae-h 组）和 66.2%（对照组）。胚胎移植后，P4D2-Ae-h 组 46.4%（116/250）的胚胎成功发育至足月，与对照组（42.9%；123/287 个胚胎）相当。总之，我们的方案（P4D2-Ae-h）在年轻的 C57BL/6J 小鼠中超数排卵是有效的。
