Ohtomo T, Yoshida K
Zentralbl Bakteriol Orig A. 1978 Dec;242(4):436-45.
Transformation for capsular-types (A and B) between four Staphylococcus aureus strains was attempted using a serum-soft agar technique to distinguish the capsular-type. DNA preparations from the Smith diffuse strain (capsular-type A) transformed strain NS58C (unencapsulated variant of capsular-type B) to type A. The strain NS58C required Ala- and His-, however, the two transformants tested were Ala+ and His+. These properties coincided with those of the donor strain just as the following characters of the transformants, which are quite different from the untransformed recipient cells: i) negative clumping factor reaction, ii) diffuse-type growth in serum-soft agar, and iii) high mouse virulence. The change to capsular-type A was antigenically comfirmed by electronmicroscopy using ferritin conjugated anti-capsular antibody.
利用血清软琼脂技术区分荚膜类型，尝试在四株金黄色葡萄球菌菌株之间进行荚膜类型（A和B）的转化。来自史密斯弥漫菌株（荚膜类型A）的DNA制剂将菌株NS58C（荚膜类型B的无荚膜变体）转化为A类型。菌株NS58C需要丙氨酸和组氨酸，然而，所测试的两个转化体为丙氨酸阳性和组氨酸阳性。这些特性与供体菌株的特性一致，就像转化体的以下特征一样，这些特征与未转化的受体细胞有很大不同：i）阴性聚集因子反应，ii）在血清软琼脂中呈弥漫型生长，iii）高小鼠毒力。使用铁蛋白偶联抗荚膜抗体通过电子显微镜在抗原上证实了向荚膜类型A的转变。
