Chomarat M, Ichiman Y, Yoshida K
Laboratoire de Bactériologie, Centre Hospitalier Lyon-Sud, France.
J Med Microbiol. 1989 Feb;28(2):129-36. doi: 10.1099/00222615-28-2-129.
Staphylococcus aureus strain MC31 showed pseudodiffuse growth in serum-soft agar and reacted with immune rabbit sera to strains Smith diffuse (capsular type A), NS58D (type B) and NS41D (type C) but not with strain NS68D (type D) in serum-soft agar. Immunisation of mice with 300 micrograms of cell-surface polysaccharide extracted from strain MC31 protected against lethal infection by strain MC31 and the strains of capsular types A, B and C. Immune rabbit serum prepared against strain MC31 passively protected mice against challenge infection with the homologous strain, but approximately 30 times more anti-MC31 serum was required to protect against infection with the strains of capsular types A, B and C. Absorption of the passive protective activity of immune sera raised against the three capsular type strains required at least 10 times the quantity of MC31 cell-surface polysaccharide than the quantity of cell-surface polysaccharide from the homologous capsular strain. Electronmicrographs of strain MC31 treated with ferritin-labelled antisera to the three capsular strains showed only small amounts of ferritin granules around the cell wall.
金黄色葡萄球菌菌株MC31在血清软琼脂中呈现假弥漫性生长，在血清软琼脂中与针对史密斯弥漫型（A 型荚膜）、NS58D（B 型）和 NS41D（C 型）菌株的免疫兔血清发生反应，但不与 NS68D（D 型）菌株反应。用从菌株 MC31 中提取的 300 微克细胞表面多糖免疫小鼠，可保护其免受菌株 MC31 以及 A、B 和 C 型荚膜菌株的致死性感染。针对菌株 MC31 制备的免疫兔血清可被动保护小鼠免受同源菌株的攻击感染，但保护小鼠免受 A、B 和 C 型荚膜菌株感染所需的抗 MC31 血清量约为前者的 30 倍。吸收针对三种荚膜型菌株产生的免疫血清的被动保护活性，所需的 MC31 细胞表面多糖量至少是同源荚膜菌株细胞表面多糖量的 10 倍。用铁蛋白标记的针对三种荚膜菌株的抗血清处理的菌株 MC31 的电子显微镜照片显示，细胞壁周围只有少量铁蛋白颗粒。
