Bengtsson A, Karlsson A, Rolfsen W, Einarsson R
Int Arch Allergy Appl Immunol. 1986;80(4):383-90. doi: 10.1159/000234085.
The allergen composition of moulds (Alternaria alternata and Aspergillus fumigatus) and mites (Dermatophagoides pteronyssinus and Dermatophagoides farinae) was studied by electroblotting. The allergens were separated by SDS-gPAGE and transferred to a nitrocellulose membrane by electroblotting. Specific IgE antibodies directed against mould and mite allergens were utilized as probes to detect the allergens. The bands were localized on the nitrocellulose membrane by means of enzyme- or isotope-labelled antibody. Circulating specific IgE antibodies in mould and mite sensitive patient sera were also analyzed and characterized with respect to their IgE specificity. Under appropriate experimental conditions this electroblotting technique could also be used for quantitation of individual allergens by scanning the isotope- or enzyme-stained nitrocellulose strips.
采用电印迹法研究了霉菌（链格孢和烟曲霉）和螨虫（粉尘螨和屋尘螨）的变应原组成。通过SDS - gPAGE分离变应原，并通过电印迹法将其转移至硝酸纤维素膜上。利用针对霉菌和螨虫变应原的特异性IgE抗体作为探针来检测变应原。通过酶标或同位素标记抗体在硝酸纤维素膜上定位条带。还对霉菌和螨虫敏感患者血清中的循环特异性IgE抗体进行了分析，并对其IgE特异性进行了表征。在适当的实验条件下，这种电印迹技术还可通过扫描同位素或酶染色的硝酸纤维素条带来定量分析单个变应原。
